姜黄素对猪脂肪间充质干细胞成脂分化及Krüppel样因子2表达的影响

doi:10.16098/j.issn.0529-1356.2017.05.007

摘要/Abstract

摘要：

目的探讨姜黄素对猪脂肪间充质干细胞(AMSCs)成脂分化的影响，及姜黄素调控成脂分化的机制。方法用不同浓度姜黄素处理猪AMSCs，用MTT比色法检测姜黄素对猪AMSCs增殖的影响，用油红O染色提取法检测对成脂分化的程度，用实时定量聚合酶链反应（Real-time PCR）检测对Krüppel样因子（KLF2）和过氧化物酶增殖物激活受体γ（PPARγ）2 mRNA表达的影响。结果当姜黄素浓度为1、5、10μmol/L用于猪AMSCs时对，AMSCs增殖无明显影响，但高于15μmol/L时，则具有显著的抑制作用（P＜0.05）；当姜黄素用于猪AMSCs的成脂诱导分化时，1~30μmol/L的浓度即能显著抑制成脂分化（P＜0.05），其中25μmol/L浓度获得68.19%的高抑制率；当25μmol/L姜黄素用于检测KLF2和PPARγ2 mRNA的表达，诱导分化2、8和16d的KLF2 mRNA的表达上调率分别达到48.37%、20.56%和9.64%，而PPARγ2 mRNA的表达下调率分别达到16.01%、35.93%和27.27%。结论姜黄素具有抑制猪AMSCs增殖和成脂分化作用，该抑制作用与姜黄素促进KLF2 mRNA的表达和抑制PPARγ2 mRNA的表达相关。

关键词: 姜黄素, Krüppel样因子2, , 脂肪间充质干细胞, 成脂分化, 实时定量聚合酶链反应, 猪

Abstract:

Objective To investigate the effects of curcumin on adipogenic differentiation and to reveal the mechanism of curcumin regulating porcine adipose mesenchymal stem cells(AMSCs) adipogenic differentiation. Methods The proliferation of porcine AMSCs was detected by MTT, the adipogenic differentiation was examined by oil red O staining extraction assay, and Krüppel like factor 2(KLF2) and peroxisome proliferator activatied receptor γ(PPARγ)2 mRNA expression patterns were detected by Real-time PCR after the porcine AMSCs were treated with various concentrations of curcumin. Results When various concentrations of curcumin were applied to treat the porcine AMSCs, 1μmol/L, 5μmol/L or 10μmol/L curcumin did not caused the significant effect on the cell proliferation, but higher than 15μmol/L curcumin had the significantly (P<0.05) inhibitory effect. The adipogenic differentiation of porcine AMSCs was detected, 1 to 30μmol/L curcumin significantly inhibited this progress, and 25μmol/L curcumin brought in the highest inhibiting rate (68.19%). When 25μmol/L curcumin was used to detect the mRNA expression patterns of KLF2 and PPARγ2 during porcine AMSCs adipogenic differentiation, the up-regulated rates of KLF2 were 48.37%, 20.56% and 9.64% and down-regulated rates of PPARγ2 were 16.01%, 35.93% and 27.27% on the day 2, day 8 and day 16 of adipogenic induction, respectively. Conclusion Curcumin inhibits the proliferation and adipogenic differentiation of porcine AMSCs, and this inhibitory effect might be associated with the upregulation of KLF2 mRNA expression and the downregulated transcription of PPARγ2.

Key words: Curcumin, Krüppel like factor 2, Adipose mesenchymal stem cell, Adipogenic differentiation, Real-time PCR, Pig

刘京霞李方正郇延军姜忠玲宋学雄. 姜黄素对猪脂肪间充质干细胞成脂分化及Krüppel样因子2表达的影响[J]. 解剖学报, 2017, 48(6): 675-681.

LIU Jing-xia LI Fang-zheng HUAN Yan-jun JIANG Zhong-ling SONG Xue-xiong. Effects of curcumin on adipogenic differentiation and expression of Krüppel like factor 2 in porcine adipose mesenchymal stem cells[J]. Acta Anatomica Sinica, 2017, 48(6): 675-681.

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