解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (6): 740-744.doi: 10.16098/j.issn.0529-1356.2018.06.008

• 组织学胚胎学发育生物学 • 上一篇    下一篇

当归多糖对D-半乳糖致小鼠胰腺损伤的保护作用

董照瀛 黄杰 许梦雄 陈雄斌 汪子铃 肖名贺 陈粼波 姜蓉 王璐 王亚平*   

  1. 重庆医科大学干细胞与组织工程研究室，组织学与胚胎学教研室，重庆 400016
  • 收稿日期:2017-12-26 修回日期:2018-04-18 出版日期:2018-12-06 发布日期:2019-02-28
  • 通讯作者: 王亚平 E-mail:ypwangcq@aliyun.com

Protective effect of Angelica polysaccharides on pancreatic injury induced by D-galactose in mice

DONG Zhao-ying HUANG Jie XU Meng-xiong CHEN Xiong-bin WANG Zi-ling XIAO Ming-he CHEN lin-bo JIANG Rong WANG Lu WANG Ya-ping*   

  1. Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China
  • Received:2017-12-26 Revised:2018-04-18 Online:2018-12-06 Published:2019-02-28
  • Contact: WANG Ya-ping E-mail:ypwangcq@aliyun.com

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摘要：

目的 探讨当归多糖（ASP）对D-半乳糖（D-gal）致小鼠胰腺损伤的保护作用。 方法 雄性C57BL/6 J 小鼠随机分为3组，每组10只。D-gal组: 小鼠皮下注射-gal (120 mg/kg， qd×42 d）； ASP+D-gal组: D-gal注射时间与剂量同D-gal模型组，从模型复制第16天起，腹腔注射ASP(40 mg/kg，qd×27 d）；正常对照组: 小鼠皮下注射等量与等时的生理盐水。各组给药完成后第2天检测相关指标，取外周血检测空腹血糖含量并行口服葡萄糖耐量测定（OGTT）；取胰腺称湿重计算脏器指数；制备石蜡切片，HE染色观察胰腺组织病理结构，图像分析法计数胰岛内有核细胞数和相关面积；制备胰腺组织匀浆，检测丙二醛(MDA)含量和总抗氧化能力（T-AOC）。 结果 从注射D-gal第16天开始，腹腔注射ASP共27 d（ASP+D-gal组），小鼠胰腺湿重与脏器指数明显降低，组织病理损伤减轻；空腹血糖显著下降；在30 min和120 min的OGTT水平差别不明显；OGTT曲线下面积（AUC）降低；胰岛内有核细胞数减少，单个细胞面积减小；T-AOC活性上升，MDA含量下降。 结论 D-gal可致胰腺结构损伤和功能衰退，ASP能拮抗D-gal从而减轻对胰腺的损伤，其机制可能与减轻氧化损伤有关。

关键词: 当归多糖, D-半乳糖, 胰腺, 损伤, HE染色, 衰老小鼠

Abstract:

Objective To explore the protective effect of Angelica sinensis polysaccharide（ASP）on murine pancreas induced by the D-galactose (D-gal). Methods The male C57BL/6J mice were randomly divided into 3 groups(10 mice in each group): D-gal group in which mice were received hypodermic injection of D-gal (120 mg/kg, qd×42 days), ASP+ D-gal group in which the dose and time of the injection of D-gal were the same as that in the D-gal group, from the 16th days of the injection of D-gal, mice intraperitoneally injected ASP(40 mg/kg，qd×27 days), and normal control group in which mice received the same dose and time of saline. The related indicators were detected on the second day after modeling and administration. Fasting blood glucose (FBG), oral glucose tolerance test (OGTT) and fasting insulin level were measured by collecting peripheral blood. Pancreas weight and visceral index were calculated. Paraffin sections were prepared. After HE staining, the micro-structures of the pancreas of each group were observed. The mononuclear cells and the related area in islets were detected by using image analysis method. Malonaldehyde (MDA) and total antioxidant capacity (T-AOC) were analyzed by preparing pancreas tissue homogenate. Results At the beginning of the 16th day of the injection of D-gal, mice were intraperitoneally injected ASP in 27 days. Compared with the control
group, pancreatic wet weight and visceral index of ASP+ D-gal mice decreased significantly. The FBG, OGTT for 30 minutes and 120 minutes had no significant difference. There was no significant difference of the blood sugar level between the groups. The area under the curve (AUC) was reduced. The number and the size of the mononuclear cells within islet decreased when compared with D-gal group. MDA content decreased, and T-AOC activity increased significantly. Conclusion D-gal can induce structural and functional injury of the pancreas. ASP can antagonise D-gal to reduce the damage to the pancreas. The mechanism may be associated with reducing oxidative damage.

Key words: Angelica sinensis polysaccharide, D-galactose, Pancreas, Injury, HE staining, Aging mouse