大鼠心肌细胞和心肌细胞系H9C2表达弹性蛋白

doi:10.16098/j.issn.0529-1356.2019.03.005

解剖学报 ›› 2019, Vol. 50 ›› Issue (3): 292-296.doi: 10.16098/j.issn.0529-1356.2019.03.005

大鼠心肌细胞和心肌细胞系H9C2表达弹性蛋白

韩贝¹ 任明芬² 常玉巧¹ 郭志坤^1*

1.新乡医学院河南省医用组织再生重点实验室，河南新乡 453003; 2.新乡医学院第二附属医院心血管内科，河南新乡 453000

收稿日期:2018-05-28 修回日期:2018-10-22 出版日期:2019-06-06 发布日期:2019-06-06
通讯作者: 郭志坤 E-mail:guozhikunxxmc@126.com
基金资助:
国家自然科学基金

Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2

HAN Bei¹ REN Ming-fen² CHANG Yu-qiao¹ GUO Zhi-kun^1*

1.He’nan Key Laboratory of Medical Tissue Regeneration; 2.Cardiovascular Department,the Second Affiliated Hospital, Xinxiang Medial University, He’nan Xinxiang 453003,China

Received:2018-05-28 Revised:2018-10-22 Online:2019-06-06 Published:2019-06-06
Contact: GUO Zhi-kun E-mail:guozhikunxxmc@126.com

摘要/Abstract

摘要：

目的检测弹性蛋白在大鼠心肌细胞和H9C2心肌细胞系内的表达。方法取新生和成年SD大鼠各5例的新鲜心脏，冷冻切片；取20只新生3 d SD大鼠心脏，通过0.05%胰蛋白酶和0.075%Ⅱ型胶原酶消化法，获取原代心肌细胞；免疫组织化学和免疫荧光技术检测大鼠心肌组织、原代心肌细胞和H9C2心肌细胞系的弹性蛋白表达;ELISA法检测培养的H9C2心肌细胞系上清中的弹性蛋白。结果在大鼠心肌细胞、H9C2心肌细胞系和新生、成年大鼠心肌组织上均发现心肌肌钙蛋白T和弹性蛋白的共表达。免疫组织化学结果发现，弹性蛋白在成纤维细胞、平滑肌细胞和细胞间质内均有表达。H9C2心肌细胞培养液的上清中检测到少量的弹性蛋白。
结论大鼠心肌细胞表达弹性蛋白，可能参与心肌细胞的势能和细胞间质弹性纤维的构建。

关键词: 弹性蛋白, 心肌细胞, 心肌细胞系H9C2, 免疫组织化学, 免疫荧光, 酶联免疫吸附测定, 大鼠

Abstract:

Objective To detect the expression of elastin in cardiomyocytes and cardiomyocyte cell line H9C2. Methods The five fresh hearts of newborn and adult SD rats are made into frozen slices.Primary cardiomyocytes were isolated from the hearts of the twenty neonatal 3 days SD rats by the digestion of 0.05% trypsin and 0.075% type Ⅱ collagenase.The technology of immunochemical and immunofluorescence were used to detect the expression of elastin in rat myocardial tissue, primary cardiomyocytes and cardiomyocyte cell line H9C2.Elastin in supernatant of cultured cardiomyocyte cell line H9C2 was detected by ELISA. Results The co-expression of cardiac troponin T and elastin was found in rat cardiomyocytes, cardiomyocyte cell line H9C2,neonatal and adult SD rat myocardial tissues.The result of immunohistochemistry also revealed the expression of elastin in fibroblasts, smooth muscle cells, and interstitial substance.A small amount of elastin was detected in the supernatant of cardiomyocyte cell line H9C2. Conclusion Rat cardiomyocytes express elastin，it may be involved in the potential energy of myocardial cells and the construction of intercellular elastic fibers.

Key words: Elastin, Cardiomyocyte, Cardiomyocyte cell line H9C2, Immunohistochemistry, Immunofluorescence, Enzyme-linked immunosorbent assay, Rat

韩贝任明芬常玉巧郭志坤. 大鼠心肌细胞和心肌细胞系H9C2表达弹性蛋白[J]. 解剖学报, 2019, 50(3): 292-296.

HAN Bei REN Ming-fen CHANG Yu-qiao GUO Zhi-kun. Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2 [J]. Acta Anatomica Sinica, 2019, 50(3): 292-296.

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大鼠心肌细胞和心肌细胞系H9C2表达弹性蛋白

Expression of elastin in rat cardiomyocytes and cardiomyocyte cell line H9C2

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