下调解耦联蛋白2表达抑制Fadu细胞增殖

doi:10.16098/j.issn.0529-1356.2019.03.006

解剖学报 ›› 2019, Vol. 50 ›› Issue (3): 297-303.doi: 10.16098/j.issn.0529-1356.2019.03.006

下调解耦联蛋白2表达抑制Fadu细胞增殖

刘颖¹李秋妍^1,3彭瑶^1,3 于海涛^2* 张春晶^1*

1. 齐齐哈尔医学院生物化学与分子生物学教研室; 2.生物遗传学教研室，黑龙江齐齐哈尔 161006; 3. 佳木斯大学生物化学与分子生物学教研室，黑龙江佳木斯 154000

收稿日期:2018-09-18 修回日期:2018-11-30 出版日期:2019-06-06 发布日期:2019-06-06
通讯作者: 于海涛;张春晶 E-mail:cjzhang2005@163.com
基金资助:
黑龙江博士后管理办公室博士后研究人员落户黑龙江科研启动资助金项目;齐齐哈尔医学院院内科研基金项目;齐齐哈尔医学院院内科研基金项目

Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation

LIU Ying¹ LI Qiu-yan ^1,3 PENG Yao^1,3 YU Hai-tao ^2* ZHANG Chun-jing^1*

1. Department of Biochemistry and Molecular Biology; 2. Department of Biology Genetics, Qiqihar Medical University, Heilongjiang Qiqihar 161006, China; 3. Department of Biochemistry and Molecular Biology, Jiamusi University, Heilongjiang Jiamusi 154000, China

Received:2018-09-18 Revised:2018-11-30 Online:2019-06-06 Published:2019-06-06
Contact: YU Hai-tao;ZHANG Chun-jing E-mail:cjzhang2005@163.com

摘要/Abstract

摘要：

目的探讨解耦联蛋白2（UCP2）对下咽癌Fadu细胞的影响，及UCP2对细胞周期以及细胞增殖的影响；同时探讨临床常用化疗药物顺铂（cisplatin）对UCP2基因下调后的Fadu细胞增殖的影响。方法流式细胞术/PI染色法检测UCP2基因对Fadu细胞周期的影响；集落形成实验检测UCP2基因下调对Fadu细胞增殖的影响；MTT法和Brdu法检测UCP2能否增强Fadu细胞对顺铂的敏感性以及使用UCP2抑制剂京平尼（genipin）处理细胞后，Fadu细胞的增殖情况。结果干扰UCP2表达抑制Fadu细胞周期于G₁/S期；抑制细胞集落形成，但干扰UCP2表达对cisplatin抑制Fadu增殖无协同作用；genipin抑制UCP2的表达后，细胞增殖受到抑制，具有时间/浓度依赖性。结论下调UCP2的表达后，Fadu细胞的增殖能力减弱，且抑制细胞周期于G₁/S期，提示UCP2可能在下咽癌Fadu细胞增殖过程中发挥重要的调节作用。

关键词: 下咽癌, 解耦联蛋白2, 顺铂, 增殖, RNA干扰技术

Abstract:

Objective The effect of uncoupling protein 2(UCP2) on the Fadu cells of the hypopharyngeal cancer was investigated in this study. The siRNA technology was used to study the effect of UCP2 on cell cycle and cell proliferation. Meanwhile, cisplatin, a commonly chemotherapeutic drug, was researched on the effect of proliferation in Fadu cells after down-regulated UCP2 gene. Methods Flow cytometry/PI staining was used to observe the effect of UCP2 on Fadu cell cycle. Clone formation assay was used to detect the effect of down-regulated UCP2 on the proliferation of Fadu cells. MTT assay and Brud assay were used to determine whether UCP2 enhanced the sensitivity of Fadu cells to cisplatin and to detect the effect of UCP2 on the proliferation of Fadu. Results The present result showed that down-regulated UCP2 expression inhibited Fadu cell cycle in G₁/S phase and cell colony formation, but had no synergistic effect on inhibition of Fadu proliferation by cisplatin; The expression of UCP2 was significantly inhibited by genipin, and then inhibited cell proliferation in a time-concentration-dependent manner. Conclusion The proliferation of Fadu cells was weakened and the cell cycle was inhibited in G₁/S phase after down-regulated UCP2, suggesting that UCP2 may play an important adjust role in the development in Fadu of hypopharyngeal cancer.

Key words: Hypopharyngeal cancer, Uncoupling protein 2, Cisplatin, Proliferation, siRNA

刘颖李秋妍彭瑶于海涛张春晶. 下调解耦联蛋白2表达抑制Fadu细胞增殖[J]. 解剖学报, 2019, 50(3): 297-303.

LIU Ying LI Qiu-yan PENG Yao YU Hai-tao ZHANG Chun-jing. Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation[J]. Acta Anatomica Sinica, 2019, 50(3): 297-303.

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下调解耦联蛋白2表达抑制Fadu细胞增殖

Down-regulated uncoupling protein 2 expression suppresses Fadu cell proliferation

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