醒神益智颗粒对血管性痴呆大鼠学习记忆能力及海马突触后致密蛋白95及突触蛋白表达的影响

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doi:10.16098/j.issn.0529-1356.2020.05.018

摘要/Abstract

摘要：

目的探讨醒神益智颗粒对血管性痴呆(VD)大鼠学习记忆能力及海马突触后致密蛋白95（PSD95）及突触蛋白（SYN）表达的影响。方法选取45只SD大鼠，采用随机数字表法分为对照组（15只）、VD组（15只）和治疗组（15只）。VD组和治疗组采用改良的间断结扎颈总动脉建立VD模型，治疗组大鼠模型制作后给予醒神益智颗粒治疗。采用Morris水迷宫实验检测3组大鼠的学习记忆能力，免疫组织化学染色检测海马CA1区PSD95及SYN蛋白的表达情况。结果与对照组大鼠相比，实验第1天、第3天及第5天，VD组大鼠逃避潜伏期［(58.3±17.9),(29.3±7.6)和(17.4±8.7)］显著提高；实验1周、4周及8周，海马PSD95［(28.25±5.09),(30.74±5.16)和(32.34±5.43)］、SYN［(12.70±6.20),(15.74±6.27)和(22.11±6.34)］蛋白水平的表达显著降低；与VD组大鼠比，实验第1天、第3天及第5天，治疗组大鼠逃避潜伏期［(55.7±17.1),(21.4±7.9)和(9.4±4.2)］显著降低；实验1周、4周及8周，治疗组海马PSD95［(35.63±3.38),(37.84±3.57)和(39.53±3.67)］及SYN［(25.75±5.79),(28.61±5.93)和(33.18±6.16)］蛋白水平的表达显著上升。与对照组相比，VD组大鼠海马SYN和PSD95表达显著减少（P<0.05）；与VD组相比，治疗组大鼠海马SYN和PSD5表达显著增加（P<0.05），但仍低于对照组。结论醒神益智颗粒可显著改善VD大鼠学习记忆功能，提高大鼠突触可塑性，其发挥神经保护作用可能与增加海马区SYN及PSD-95的表达相关。

关键词: 醒神益智颗粒, 血管性痴呆, 学习记忆能力, 突触后致密蛋白95, 突触蛋白;, 免疫组织化学, 大鼠

Abstract:

Objective To study the effects of Xingshen Yizhi granule on the learning and memory ability and the expression of postsynaptic dense protein 95 (PSD95) and synapsin (SYN) protein in the hippocampus of vascular dementia (VD) rats.

Methods Totally forty-five SD rats were randomly divided into control group (n=15),VD group (n=15)and treatment group (n=15).VD rat model was established in the VD group and treatment group with modified intermittent ligation of common carotid artery, and the rats in the treatment group were treated with Xingshen Yizhi granule after membrane formation. The learning and memory ability of three groups of rats were tested by Morris water maze test. The expression of PSD95 and SYN in hippocampal CA1 was detected by immunohistochemical staining. Results Compared with the control group SD rats, at day 1, day 3 and day 5 of the experiment, the escape latency of SD rats in VD group ［(58.3±17.9), (29.3±7.6) and (17.4±8.7)］ increased significantly［(58.3±17.9), (29.3±7.6) and (17.4±8.7)］. At week 1, week 4 and week 8, the expression of hippocampal PSD95 ［(28.25±5.09), (30.74±5.16) and (32.34±5.43)］ and SYN ［(12.70±6.20), (15.74±6.27) and (22.11±6.34］ protein decreased significantly. Compared with the VD group, at day 1, day 3 and day 5 of the experiment, the escape latency of SD rats in the treatment group ［(55.7±17.1), (21.4±7.9) and (9.4±4.2)］ decreased significantly. At week 1, week 4 and week 8, the expression of hippocampal PSD95 in the treatment group ［(35.63±3.38), (37.84±3.57) and (39.53±3.67)］,SYN ［(25.75±5.79), (28.61±5.93) and (33.18±6.16)］ protein increased significantly. Compared with the control group, the expression of SYN and PSD95 in the hippocampus of the model group decreased significantly (P<0.05).Compared with the model group, the expression of SYN and PSD95 in the hippocampus of the treatment group was significantly higher(P<0.05), but it was still lower than that of the control group.

Conclusion Xingshen Yizhi granule can significantly improve learning and memory function and increase synaptic plasticity in VD rats. Its neuroprotective effect may be related to the increased expression of SYN and PSD95 in the hippocampus.

Key words: Xingshen Yizhi granule, Vascular dementia, Learning and memory ability, Postsynaptic dense protein 95, Synapsin, Immunohistochemistry, Rat

中图分类号:

R365.749

吴世卫关建军陈瑜. 醒神益智颗粒对血管性痴呆大鼠学习记忆能力及海马突触后致密蛋白95及突触蛋白表达的影响

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[J]. 解剖学报, 2020, 51(5): 745-750.

WU Shi-wei GUAN Jian-jun CHEN Yu. Effects of Xingshen Yizhi granule on the learning and memory ability and the expression of postsynaptic dense protein 95 and synapsin protein in the hippocampus of vascular dementia rats[J]. Acta Anatomica Sinica, 2020, 51(5): 745-750.

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醒神益智颗粒对血管性痴呆大鼠学习记忆能力及海马突触后致密蛋白95及突触蛋白表达的影响
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Effects of Xingshen Yizhi granule on the learning and memory ability and the expression of postsynaptic dense protein 95 and synapsin protein in the hippocampus of vascular dementia rats

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