银杏叶提取物对青春期乏运动大鼠行为学的影响

doi:10.16098/j.issn.0529-1356.2021.01.005

解剖学报 ›› 2021, Vol. 52 ›› Issue (1): 36-40.doi: 10.16098/j.issn.0529-1356.2021.01.005

银杏叶提取物对青春期乏运动大鼠行为学的影响

程晶¹ 游剑虹² 陈琳琳³李振喜^1*

1.福建省泉州医学高等专科学校基础医学部，福建泉州362000; 2.厦门大学附属中山医院超声科，福建厦门361000; 3.福建省泉州医学高等专科学校药学院，福建泉州362000

收稿日期:2019-10-09 修回日期:2019-11-26 出版日期:2021-02-06 发布日期:2021-02-06
通讯作者: 李振喜 E-mail:83186698@qq.com
基金资助:
泉州市科技计划项目

Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness

CHENG Jing¹YOU Jian-hong² CHEN Lin-lin³ LI Zhen-xi^1*

1.Department of Basic Medicine, Quanzhou Medical College, Fujian Quanzhou362000, China; 2.Department of Ultrasound, Zhongshan Hospital of Xiamen University, Fujian Xiamen361000,China; 3.Department of Pharmacy, Quanzhou Medical College, Fujian Quanzhou362000,China

Received:2019-10-09 Revised:2019-11-26 Online:2021-02-06 Published:2021-02-06
Contact: LI Zhen-xi E-mail:83186698@qq.com

摘要/Abstract

摘要：

目的探讨银杏叶提取物对青春期乏运动大鼠行为学的影响及其可能机制。方法将24只4周龄SD雄鼠随机分为正常对照组(NC）、乏运动组（AS）以及银杏叶提取物处理组（GBE），干预性饲养至8周龄，进行旷场实验及高架十字迷宫实验检测行为学改变。比色法检测脑组织匀浆丙二醛（MDA）含量及超氧化物歧化酶(SOD)活性。Western blotting 检测脑实质糖原合成激酶3β(GSK-3β)和 β-连环蛋白(β-catenin)蛋白表达水平。结果旷场实验显示，AS组大鼠自主活动增加，而在旷场中央区域停留时间及次数减少；高架十字迷宫实验显示，AS组大鼠进入开臂时间及次数均减少。GBE干预有效逆转AS诱导的焦虑样行为（P＜0.05）。与NC组相比，AS组GSK-3β 和β-catenin比值上调，MDA含量增加，SOD活性降低；而GBE组GSK-3β和β-catenin比值明显下调，MDA含量下降，SOD活性增加（P＜0.05）。结论银杏叶提取物对青春期乏运动大鼠焦虑样行为具有改善作用，Wnt/β-catenin 信号通路及抗氧化调节可能参与脑保护作用。

关键词: 银杏叶提取物, 运动缺乏, 焦虑样行为, Wnt/β-连环蛋白信号通路, 免疫印迹法, 大鼠

Abstract:

Objective To investigate the effect of Ginkgo biloba extract on behaviors in rat with adolescent sedentariness and its possible mechanism. Methods Twenty-four male SD rats (4-week-old) were randomly divided into normal control (NC), adolescent sedentarines (AS), and Ginkgo biloba extract (GBE) groups. After 4 weeks intervention with GBE, open-field test and elevated plus-maze test were performed to detect the behavioral changes. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD)in brain were determined by colorimetric method. Protein levels of glycogen synthase kinase 3β(GSK-3β) and β-catenin in cerebral white matter were determined by Western blotting. ResultsIn open-field test, it was shown that the autonomic activity of rats in AS group increased, while the central regional travel time was reduced. Duration and number of residence in the open arm of elevated plus-maze test decreased in AS group. These anxiety-like behaviors were ameliorated by GBE intervention. Compared with the NC group, GSK-3β/β-catenin ratio and the content of MDA was upregulated in AS group while downregulated in GBE group. And activity of SOD were downregulated in AS group while significantly upregulated in GBE group (P<0.05). Conclusion Ginkgo biloba extract ameliorate anxiety-like behavior in rat with adolescent sedentariness. Wnt/β-catenin pathway and antioxidant regulation may play a cerebral protective role.

Key words: Ginkgo biloba extract, Physical inactivity, Anxiety-like behavior, Wnt/β-catenin pathway, Western blotting, Rat

中图分类号:

R741.05，R964

程晶游剑虹陈琳琳李振喜. 银杏叶提取物对青春期乏运动大鼠行为学的影响[J]. 解剖学报, 2021, 52(1): 36-40.

CHENG Jing YOU Jian-hong CHEN Lin-lin LI Zhen-xi. Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness[J]. Acta Anatomica Sinica, 2021, 52(1): 36-40.

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银杏叶提取物对青春期乏运动大鼠行为学的影响

Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness

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