小鼠动情周期内雌孕激素波动与皮层及海马 δ-亚基的突触外γ-氨基丁酸A型受体表达的相关性

doi:10.16098/j.issn.0529-1356.2021.06.001

解剖学报 ›› 2021, Vol. 52 ›› Issue (6): 839-844.doi: 10.16098/j.issn.0529-1356.2021.06.001

• 神经生物学 • 下一篇

小鼠动情周期内雌孕激素波动与皮层及海马 δ-亚基的突触外γ-氨基丁酸A型受体表达的相关性

郑小敏^1,2,3* 张庭元⁴卜超志¹ 黄璐¹ 周涛¹ 叶扬¹ 张怡璇¹ 任勇威¹ 江世文^1*

1.无锡市妇幼保健院优生优育遗传医学研究所妇产科, 江苏无锡 214000; 2.宁夏医科大学基础医学院人体解剖学与组织学胚胎学系,生育力保持省部共建教育部重点实验室, 银川 750004; 3.赫尔辛基大学医学研究中心，赫尔辛基 00014，芬兰; 4.中国人民解放军空军医学特色中心妇产科，北京 100142

收稿日期:2020-06-29 修回日期:2020-09-22 出版日期:2021-12-06 发布日期:2021-12-06
通讯作者: 郑小敏;江世文 E-mail:20070034@nxmu.edu.cn
基金资助:
突触外GABAA受体与生殖周期相关抑郁症发生的研究;江苏省青年医学人才计划;无锡市妇幼保健院院级基金项目

Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice

ZHENG Xiao-min^1,2,3* ZHANG Ting-yuan⁴ BU Chao-zhi¹ HUANG Lu¹ ZHOU Tao¹ YE Yang¹ ZHANG Yi-xuan¹ REN Yong-wei¹ JIANG Shi-wen^1*

1.Institute of Prenatal and Childbearing Genetic Medicine, Department of Obstetrics and Gynecology, Wuxi Maternity and Child Health Hospital, Jiangsu Wuxi 214002, China;2.Department of Human Anatomy and Embryology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China; 3.Institute of Biomedicine, University of Helsinki, Helsinki 00014, Finland;4.Obstetrics and Gynecology, Air Force General Hospital, PLA, Beijing 100142, China

Received:2020-06-29 Revised:2020-09-22 Online:2021-12-06 Published:2021-12-06
Contact: ZHENG Xiao-min;JIANG Shi-wen E-mail:20070034@nxmu.edu.cn

摘要/Abstract

摘要：

目的探讨雌性小鼠动情周期不同阶段血清孕酮浓度与大脑皮质和海马中含δ-亚基的突触外γ-氨基丁酸A型受体（δGABA_ARs）表达的相关性。方法动情间期和动情期成熟卵巢周期雌性小鼠（4～6周，n=12）血清雌激素和孕酮浓度采用ELISA试剂盒检测；动情间期和动情期大脑皮层和海马中δGABA_ARs表达采用免疫组织化学方法检测。结果在动情间期，皮层和海马δGABA_ARs表达明显高于动情期，血清孕酮的浓度与δGABA_ARs的积分吸光度值成线性关系。结论动情期不同阶段孕酮的生理波动会影响δGABA_A Rs在海马及皮层等特定脑区的表达，而海马神经元δGABA_ARs表达的差异会影响脑区神经元的兴奋度，从而可能影响雌性动物的行为特性。

关键词: γ-氨基丁酸A型受体, 动情周期, 黄体酮, 皮层, 海马, 免疫组织化学, 小鼠

Abstract:

Objective To explore the correlation of progesterone and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors (δGABA_ARs) in cerebral cortex and hippocampus， during different phase of estrus cycles in female mice. Methods Serum concentrations of estrogen and progesterone，of well-established ovarian cycles in female mice (4-6weeks, n=12) in estrus and di-estrus, had detected by ELISA kit. Immunohistochemistry was used to mark the difference expression of δGABA_ARs expression, in cerebral cortex and hippocampus during these two phases. Results The expression of δGABA_ARs was significant higher in di-estrus than estrus in hippocampus. The concentration of serum progesterone had co-inearity with integrated absorbance of δGABA_ARs in different phase of female estrus at the same brain region. Conclusion The physiological fluctuations of progesterone at different stages of estrus affect the expression of δGABA_ARs in the hippocampus and cortex and other specific brain regions. The differences in the expression of δGABA_ARs in hippocampal neurons will affect the excitability of brain neurons, which may further affect the behavioral characteristics of female animals.

Key words: γ-aminobutyric acid type A receptor, Estrus cycle, Progesterone, Cortex, Hippocampus, Immunohistochemistry, Mouse

中图分类号:

Q593⁺2

郑小敏张庭元卜超志黄璐周涛叶扬张怡璇任勇威江世文 . 小鼠动情周期内雌孕激素波动与皮层及海马 δ-亚基的突触外γ-氨基丁酸A型受体表达的相关性[J]. 解剖学报, 2021, 52(6): 839-844.

ZHENG Xiao-min ZHANG Ting-yuan BU Chao-zhi HUANG Lu ZHOU Tao YE Yang ZHANG Yi-xuan REN Yong-wei JIANG Shi-wen. Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice[J]. Acta Anatomica Sinica, 2021, 52(6): 839-844.

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小鼠动情周期内雌孕激素波动与皮层及海马 δ-亚基的突触外γ-氨基丁酸A型受体表达的相关性

Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice

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