解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (6): 870-874.doi: 0.16098/j.issn.0529-1356.2021.06.005

• 神经生物学 • 上一篇    下一篇

MCC950下调即早基因的表达缓解甲醛所致小鼠炎性痛

李威1 赵可心1 周鑫1 谢志艳1 曾学晴1 袁婧1 钟小林2  万炜1 杨惠3*  曹文宇1* 
  

  1. 1.南华大学衡阳医学院应用解剖学与生殖医学研究所，湖南 衡阳 421001;  2.南华大学附属第一医院内分泌研究所，湖南 衡阳 421001;  3.南华大学附属第一医院病理科，湖南 衡阳 421001
  • 收稿日期:2020-03-06 修回日期:2020-05-14 出版日期:2021-12-06 发布日期:2021-12-06
  • 通讯作者: 杨惠;曹文宇 E-mail:1103177063@qq.com
  • 基金资助:
    湖南省教育厅优秀青年项目;湖南省自然科学基金青年项目;南华大学大学生研究性学习和创新性实验计划项目;南华大学大学生研究性学习和创新性实验计划项目;南华大学大学生研究性学习和创新性实验计划项目;南华大学大学生研究性学习和创新性实验计划项目

MCC950 alleviating inflammatory pain induced by formaldehyde through down-regulation of immediate early genes in mouse spinal cord

LI Wei1  ZHAO Ke-xin1  ZHOU Xin1  XIE Zhi-yan1  ZENG Xue-qing1  YUAN Jing1  ZHONG Xiao-lin2  WAN Wei1  YANG Hui3*  CAO Wen-yu1*   

  1. 1.Clinical Anatomy and Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hu’nan Hengyang 421001, China; 2.Institute of Clinical Endocrine, the First Affiliated Hospital of University of South China, Hu’nan Hengyang 421001, China; 3.Institute of Clinical Pathology, the First Affiliated Hospital of University of South China, Hu’nan Hengyang 421001, China
  • Received:2020-03-06 Revised:2020-05-14 Online:2021-12-06 Published:2021-12-06
  • Contact: YANG Hui;CAO Wen-yu E-mail:1103177063@qq.com

PDF

摘要：

目的  探讨MCC950对甲醛模型小鼠疼痛的影响及可能机制。   方法  72只ICR雄性小鼠随机分为生理盐水对照组（NS组），甲醛模型组（F组）、不同浓度梯度MCC950干预组（6.25 mg/kg MCC950+F、12.5 mg/kg MCC950+F、25 mg/kg MCC950+F、50 mg/kg MCC950+F）。以小鼠累计舔咬足时间评价自发痛行为；采用Real-time PCR方法检测小鼠脊髓即早基因，包括c-Fos、Arc、早期生长反应因子-1（Egr-1）mRNA的表达变化；采用免疫组织化学法检测脊髓背角内c-Fos的表达。   结果  自发痛评分显示，与NS组相比，F组小鼠出现典型双相痛；与F组相比，12.5 mg/kg、25 mg/kg及50 mg/kg MCC950预处理均可显著缓解甲醛所致小鼠疼痛。Real-time PCR结果显示，与NS组相比， F组小鼠脊髓早期基因（c-Fos、Arc、Egr-1）的mRNA表达均上调，50 mg/kg MCC950预处理后，上述指标的mRNA表达均显著下调。免疫组织化学结果显示，与NS组相比，F组小鼠脊髓背角c-Fos表达明显增加，而用50 mg/kg MCC950预处理后，小鼠脊髓背角c-Fos表达下降。   结论  MCC950可有效缓解甲醛所致小鼠炎性痛，其机制可能与下调脊髓即早基因（c-Fos、Arc、Egr-1）的表达有关。

关键词: MCC950, 甲醛, 炎性痛, 脊髓, 即早基因, 实时定量聚合酶链反应, 小鼠

Abstract:

Objective  To investigate the effect of MCC950 on inflammatory pain induced by formaldehyde in mice.    Methods  Seventy-two male ICR mice were randomly divided into six groups, including saline control group (NS), formaldehyde model group(F), different doses of MCC950 intervention group (6.25 mg/kg MCC950+F, 12.5 mg/kg MCC950+F, 25 mg/kg MCC950+F, 50 mg/kg MCC950+F). The spontaneous pain was determined by the cumulative time of licking paw in mice. The expressions of immediate early genes (IEGs), such as c-Fos, Arc, early growth response protein 1(Egr-1), in the spinal cord were determined by Real-time PCR. The expression of c-Fos in the spinal cord was determined by immunohistochemistry.    Results  Spontaneous pain score showed that compared with the NS group，F group showed typical biphasic pain, however 12.5 mg/kg, 25 mg/kg and 50 mg/kg MCC950 intervention decrease significantly the second phase pain compared with the F group. The result of Real-time PCR showed that the mRNA level of c-Fos, Arc and Egr-1 in the spinal cord of  F group enhanced remarkably compared with the NS group, while it could be reversed by 50 mg/kg MCC950 treatment. The result  of immunohistochemistry showed that the number of c-Fos positive cells in the posterior horn of spinal cord of the F group increased significantly compared with the NS group, while it could be reversed by the treatment with 50 mg/kg MCC950.    Conclusion  MCC950 can alleviate the inflammatory pain induced by formaldehyde in mice, which may be related to the down-regulation of IEGs in the spinal cord.

Key words: MCC950, Formaldehyde, Inflammatory pain, Spinal cord, Immediate early gene, Real-time PCR, Mouse

中图分类号: