›› 2007, Vol. 38 ›› Issue (1): 52-55.doi:
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胡慧敏;陈先文*;施雪英;徐仿成
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关键词: 孤儿核受体, 骨髓基质细胞, 三七总皂甙, 全反式维甲酸, 免疫细胞化学, 大鼠
Abstract: Objective To study the effect of Nurrl gene on the differentiation of rats’ marrow stromal cells (MSCs) into neurous under the coinducement of total panax notoginserg saponins (tPNS) and alltransretineic acid (ATRA) by coloning the Nurrl gene and transfecting it into MSCs. Methods Expressing plasmids pcDNA31hygroNurrl were cloned, then transfected into MSCs with lipofectamine 2000. To begin with, MSCs were subcultured into 6wells cultured plate at about 5×105 cells/well density and the wells were divided into four groups randomly which were Nurrl+tPNS/ATRA group, tPNS/ATRA group, Nurrl group and control group. Secondly, the plasmids were introduced to the MSCs in Nurrl+tPNS/ATRA group and Nurrl group,then protein expression of Nurrl was identified with immunocytochemistry. Thirdly, after the MSCs and plasmids had been cocultured for 48 hours, cells in Nurrl+tPNS/ATRA group and tPNS/ATRA group were induced with BME in advance then with tPNS/ATRA in due form. For cells in Nurrl and control group, the only difference was that tPNS/ATRA was replaced with the culture. Finally we compared the different percentage of positive cells in four groups with TH, AChE and GABA antibodies by immunocytochemistry method. Results The immunocytochemical test showed that the MSCs transfected with Nurrl gene expressed Nurrl protein. The percentage of positive cells of TH antibody in Nurrl+tPNS/ATRA group was (38.4±4.6)% distinctly higher than that of tPNS/ATRA group, which was (5.9±3.4)%. Conclusion With tPNS/ATRA induced and immunocytochemistry of TH, positive cells percentage in N
Key words: P>Orphan nuclear receptor, Marrow stromal cells, tPNS, ATRA, Immunocytochemistry, Rat/P>
中图分类号:
Q421,R338
胡慧敏;陈先文;施雪英;徐仿成. 转染Nurrl基因的大鼠骨髓基质细胞的诱导分化[J]. , 2007, 38(1): 52-55.
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https://jpxb.bjmu.edu.cn/CN/Y2007/V38/I1/52
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