解剖学报

解剖学报 ›› 2023, Vol. 54 ›› Issue (5): 593-598.doi: 10.16098/j.issn.0529-1356.2023.05.014

• 组织学胚胎学发育生物学 • 上一篇    下一篇

发生发育小鼠肾输尿管芽分支的形态特点

 顾玲1 张萍2 宋科昕1 李娜3 陈虹宇1 张婕1 丛敬4* 翟效月1*    

  1. 1.中国医科大学基础医学院组织学与胚胎学教研室，沈阳 110122； 2.锦州医科大学基础医学院组织学胚胎学教研室，辽宁 锦州 121001； 3.沈阳市第四人民医院干诊科，沈阳 110082；4.沈阳医学院基础医学院组织学胚胎学教研室，沈阳 110034

  • 收稿日期:2022-05-08 修回日期:2022-06-17 出版日期:2023-10-06 发布日期:2023-12-25
  • 通讯作者: 翟效月 E-mail:xyzhai@cmu.edu.cn
  • 基金资助:
    国家自然科学基金

 Morphometry of the ureteric bud branching in the developing mouse kidney

GU Ling1  ZHANG Ping2  SONG Ke-xin1  LI Na3  CHEN Hong-yu1  ZHANG Jie1 CONG -Jing4*  ZHAI Xiao-yue1*    

  1. 1.Department of Histology and Embryology, College of Basic Medical Science, China Medical University, Shenyang 110122, China;  2.Department of Histology and Embryology, College of Basic Medical Science, Jinzhou Medical University, Liaoning Jinzhou 121001, China;   3.Department of Geriatric Medicine, the Fourth People’s Hospital of Shenyang, Shenyang 110082, China; 4.Department of Histology and Embryology, College of Basic Medical Science, Shenyang Medical College, Shenyang 110034, China
  • Received:2022-05-08 Revised:2022-06-17 Online:2023-10-06 Published:2023-12-25
  • Contact: ZHAI XiaoYue E-mail:xyzhai@cmu.edu.cn

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摘要：

目的  通过对输尿管芽树状结构三维追踪，结合形态学分析及测量，探讨输尿管芽分支模式及其末端诱导肾单位发生的数量。  方法  取胚胎期及生后多时间点小鼠肾脏各3只，制备石蜡和树脂连续切片，显微图像摄取并配准，计算机辅助输尿管芽追踪并可视化，计数其分支数及单个分支所诱导的肾单位数量。此外，石蜡切片进行HE染色用于生肾区与输尿管芽形态学观察，Claudin-7免疫组织化学染色标记输尿管芽，测量输尿管芽分支末端的数量及反映其密度的输尿管芽间距。  结果  输尿管芽树状分支三维可视化显示，起始的两主叉分支构成肾髓质，而皮质和生肾区分支茂密，输尿管芽分支末端间距越来越小。单个输尿管芽末端诱导肾单位数量由胚胎14.5 d的1个到胚胎17.5 d的2个，偶见3个。   结论  三维可视化显示输尿管芽树状分支呈区域复杂性，提示不同区域分子驱动不同的分支模式；而输尿管芽末端在生肾区的密度随肾脏发生发育而增加，与生肾区厚度减少相一致，而出生后分支末端的消失也反映了与肾单位祖细胞逐渐耗竭相一致。 

关键词:  肾脏, 输尿管芽, 分支, 肾单位数量, 发生发育, 三维可视化, 小鼠 
 

Abstract:

 Objective  To investigate the branching pattern of the ureteric bud and the number of the nephron induced by each ureteric bud tip, through the three-dimensional tracing of the ureteric tree, combined with the morphological analysis and measurement of the ureteric tree.    Methods  The kidneys were obtained from three mice at various developing time points and prepared for paraffin and epoxy sections. Then the microscopic images were digitized and aligned from these sections. Based on the computerassisted tracing and visualization of ureteric tree, the number of branches and the nephron induced by each ureteric bud tip were obtained by counting. In addition, paraffin sections were stained with HE staining for morphological observation of nephrogenic zone and ureteric bud, while in order to reflect the density of the ureteric bud tips at nephrogenic zone, the distance between two neighboring ureteric bud tips was measured aided with the Claudin-7 immunohistochemical staining.    Results  The ureteric bud branching tree revealed that the initial bifid iterative branching formed the framework of renal medulla, the branching became complicated and dense in cortex and nephrogenic zone, while the distance between ureteric bud tips were also decreasing. The number of the nephron induced by each ureteric bud tip increased from one (E14.5) to two (E17.5), and occasionally to three.  Conclusion  Three-dimeasional Visualization of ureteric bud branching tree reveals regional complication, suggesting molecules in different regions drive different branching patterns; While the density of the ureteric bud tips at nephrogenic zone increases corresponding to decreasing of thickness of the nephrogenic zone, and the disappearance of the ureteric bud tips after birth is also consistent with the gradual consumption of nephron progenitor cells. 

Key words:  Kidney, Ureteric bud, Branching, Nephron number, Development, Three-dimensional visualization, Mouse
 

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