鸡肠神经胶质细胞超微结构特征及其标记蛋白钙结合蛋白S100β在小肠中的分布

摘要/Abstract

摘要： 目的观察了10只健康黄羽肉鸡肠神经胶质细胞结构特征及S-100β蛋白的分布特点。方法采用透射电镜技术，观察神经胶质细胞的超微结构特点，免疫组织化学SABC-AP法研究S-100β蛋白的分布特征。结果电镜观察表明鸡肠神经胶质细胞（EGCs）在小肠各段均呈星形，细胞核不规则，胞质中分布有大小不一的圆形或椭圆形无髓神经纤维。免疫组织化学显示，S-100β在鸡小肠各段黏膜上皮细胞、肠腺表达较强，其中，上皮细胞基膜和肠腺上皮细胞顶端为强阳性，固有膜表达较弱，在黏膜下神经丛和肌间神经丛均呈强阳性表达。结论鸡肠神经胶质细胞超微结构特征及其在小肠的分布特点与哺乳动物较为相似。

关键词: 肠神经胶质细胞, 超微结构, S-100β, 小肠, 免疫组织化学, 鸡

Abstract: objective To observe the structure of Enteric glial cell and distribution of S-100β protein in 10 healthy yellow feather broiler chickens. Methods The ultrastructural characteristics of glial cells were observed by transmission electron microscopy， and the distribution of S-100 protein was studied by immunohistochemical SABC-AP method. Results The electron microscope showed that chicken enteric glial cells (EGCS) in the small intestine were star， irregular nucleus， cytoplasm distribution has a small round or oval unmyelinated nerve fibers. Immunohistochemistry showed that in chicken intestine epithelium mucosa of each segment， intestinal glands of S-100βwere expressed strong. Among the basement membrane of epithelial cells and at the top of intestinal gland epithelial cells were the strong positive， lamina propria weaker expression， in the submucosal plexus and myenteric plexus showed strong positive expression. Conclusion the glial cell ultrastructure characteristics and distribution characteristics of S-100βare similar to the small intestine of mammals.

Key words: Enteric glial cell, Ultrastructural, S-100β, the small intestine, immunohistochemistry, chicken

徐春生祁凤华摆世林陈城蔡婷婷. 鸡肠神经胶质细胞超微结构特征及其标记蛋白钙结合蛋白S100β在小肠中的分布[J]. , 2016, 47(1): 7-11.

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