解剖学报

›› 2010, Vol. 41 ›› Issue (4): 631-633.doi: 10.3969/j.issn.0529-1356.2010.04.030

• 技术方法 • 上一篇    

细胞膜红色荧光探针（DiI）光学转换及其电镜样本的制备

崔占军1，2; 赵凯冰3; 牛艳丽1 ;邓锦波1*    

  1. 1.河南大学神经生物学研究所;2.河南大学医学院人体解剖学教研室;3.开封大学医学部人体解剖教研室，河南 开封 475001
  • 收稿日期:2009-06-12 修回日期:2009-07-14 出版日期:2010-08-06
  • 通讯作者: 邓锦波

Photoconvertion of DiI and the preparation of the electron microscope sample

  1. 1.Institute of Neurobiology， He’nan University; 2.Department of Anatomy, Medical College of He′nan University;3.Department of Anatomy, Medical College of Kaifeng University ，He′nan Kaifeng 475001，China
  • Received:2009-06-12 Revised:2009-07-14 Online:2010-08-06
  • Contact: DENG Jin-bo

关键词: 神经元, DiI散射标记, 光学转换, 超微结构, C57/B6J小鼠

Abstract: Objective In order to make the labeled neurons and glia with 1,1′-dioctadecy-3,3,3′,3,-tetramethylindocarbocyanine perchlorate(DiI) diolistic labeling for long time preservation and ultrastructural study. Methods DiI diotistic assays were done on the sections of visual cortex of 20 C57/B6J mice to label neurons and glia, then the targeted neurons were photoconverted and prepared for ultrathin section. Results The neurons after the photoconversion could still show fine shape with spines on dendrites and decent synapse ultrastructure. Conclusion After DiI diotistic labeling and photoconversation, the labeled neurons can present satisfied morphological details with either light microscopy or electron microscopy. The organelle is well and the synaptic structure is clear either.

Key words: Neuron, DiI diotistic labeling, Photoconvertion, Ultrastructure, C57/B6J mouse

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