解剖学报

›› 2008, Vol. 39 ›› Issue (6): 877-880.doi:

• 论著 • 上一篇    下一篇

硫化氢对体外大鼠肝星状细胞内Ca2+浓度变化及细胞增殖的影响

尚宏伟1 ;王利军2 ;王兴翠1 ;尤红3 ;唐淑珍3 ;高尔静1 ;丁惠国2*; 贾继东3   

  1. 1. 首都医科大学基础医学院组织学胚胎学教研室; 2.首都医科大学附属北京佑安医院肝病消化科;3.首都医科大学附属北京友谊医院肝病中心，北京 100069
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-06
  • 通讯作者: 丁惠国

EFFECTS OF HYDROGEN SULFIDE ON INTRACELLULAR CALCIUM AND PROLIFERATION IN HEPATIC STELLATE CELLS EM>IN VITRO/EM>

  1. 1. Department of Histology and Embryology, Basic Medical Science College of Capital Medical University;2.Department of Gastroenterology and Hepatology, Beijing Youan Hospital Affiliated to Capital Medical University;3. Centre of Hepatology, Friendship Beijing Hospital Affiliated to Capital Medical University, Beijing 100069, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-06
  • Contact: DING Hui-guo

关键词: 硫化氢, 肝星状细胞, 四唑盐比色法, 激光扫描共焦显微镜, 细胞培养, 大鼠

Abstract: Objective To study the effect of hydrogen sulfide (HSUB>2/SUB>S) on intracellular calcium concentration and proliferation in hepatic stellate cells (HSC) and the possible mechanism of HSUB>2/SUB>S. Methods HSC-T6, an activated rat HSC, was plated in 35-mm culture dish at a density of 1×10SUP>5/SUP>/ml, and rotuinely incubated in DMEM media for 24 hours. The intracellular CaSUP>2+/SUP> loaded by Flu-3/AM was measured by laser scanning confocal microscope. The dynamic changes of intracellular CaSUP>2+/SUP> fluorescence intensity(［CaSUP>2+/SUP>］i ) stimulated by NaSH, a HSUB>2/SUB>S donor, were measured. The HSC proliferations were measured by MTT at different HSUB>2/SUB>S concentrations. Results Low concentration of HSUB>2/SUB>S(100μmol/L) decreased significantly HSC-T6 ［CaSUP>2+/SUP>］i (16.20±3.56 vs 14.12±3.76,EM>P/EM><0.05). The increased HSC-T6 proliferations were also observed in low concentration stimulation of HSUB>2/SUB>S(50-100μmol/L). The decreased ［CaSUP>2+/SUP>］i was inhibited by glibenclamide, a KSUB>ATP/SUB> channel antagonist， significantly when administered with HSUB>2/SUB>S of higher concentrations. However, high concentration of HSUB>2/SUB>S(1mmol/L) increased significantly HSC-T6 ［CaSUP>2+/SUP>］i，P<0.05, with no significant changes in proliferation. Conclusion Th

Key words: Hydrogen sulfide, Hepatic stellate cell, MTT, Laser scanning confocal microscope, Cell culture, Rat

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