解剖学报

›› 2009, Vol. 40 ›› Issue (5): 840-843.doi: 10.3969/j.issn.0529-1356.2009.05.031

• 技术方法 • 上一篇    下一篇

两种显示大鼠不同组织微血管方法的比较

张爽1,2; 张涛3 ;王磊1; 王安如1; 俞英昉1; 滕可导1*   

  1. 1. 中国农业大学动物医学院，北京 100193; 2.中国科学院动物研究所，北京 100101; BR>3. 北京农学院兽医学中医药北京市重点实验室，北京 102206
  • 收稿日期:2008-08-11 修回日期:2008-10-13 出版日期:2009-10-06
  • 通讯作者: 滕可导

Comparison between two methods for displaying microvessels in rat different tissues

  1. 1.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; 2.Institute of Zoology, Chinese Academy of Sciences, Beijing 100101,China;3.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing 102206, China
  • Received:2008-08-11 Revised:2008-10-13 Online:2009-10-06
  • Contact: TENG Ke-dao

关键词: 微血管, CD34, 硝基四氮唑蓝/5-溴-4-氯-3-吲哚磷酸盐, 免疫组织化学, 酶组织化学, 大鼠

Abstract: bjective To investigate the differences between CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry in displaying microvessels of various tissues. Methods The microvessels in rat small intestinal, kidney, spleen, liver, heart, lung and skin were stained by CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry, and the two methods were compared by morphological analysis and microscopic count. Results We found that the microvessels in all selected tissues above mentioned were stained by CD34 immunohistochemistry, and that the microvessels in small intestinal, kidney, liver and heart tissues, but not in spleen, lung and skin tissues, were stained by NBT/BCIP enzymic histochemistry. And in the same sections of one tissue, the microvascular density by NBT/BCIP enzymic histochemistry was higher than that by CD34 immunohistochemistry. Conclusion CD34 immunohistochemistry is generally suitable to display the microvesssels in various organs, while NBT/BCIP enzymic histochemistry shows some organ microvessels at higher density.

Key words: Microvessel, CD34, NBT/BCIP, Immunohistochemistry, Enzymic histochemistry, Rat

中图分类号: