解剖学报

›› 2009, Vol. 40 ›› Issue (2): 256-259.doi: 10.3969/j.issn.0529-1356.2009.02.017

• 论著 • 上一篇    下一篇

乙醛脱氢酶2抑制剂大豆甙对缺氧心肌细胞的MAPK信号转导作用

徐丹令1，2 ;孙爱军1 ;王时俊1 ;王翔飞1 ;贾剑国1;顾兴华1 ;王克强1 ;邹云增1 ;葛均波1*   

  1. 1.复旦大学附属中山医院上海市心血管病研究所，上海 200032; 2.同济大学医学院 人体解剖学教研室，上海 200092
  • 收稿日期:2008-01-14 修回日期:2008-03-19 出版日期:2009-04-06
  • 通讯作者: 葛均波

Study of ALDH2 inhibitor daidzin role on rat myocytes MAPK singaling pathway under hypoxia

  1. 1.Shanghai Insititute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China;2. Department of Anatomy, Medical College of Tongji University, Shanghai 200092, China
  • Received:2008-01-14 Revised:2008-03-19 Online:2009-04-06
  • Contact: GE Jun-bo

关键词: 乙醛脱氢酶2, 缺氧, 心肌, 大豆甙, 丝裂原活化蛋白激酶, Western blotting, 大鼠

Abstract: Objective To observe the role of ALDH2 on cardiac myocytes variation of reactive oxygen species (ROS), apoptosis and mitogen-activated protein kinases (MAPK) signal route under hypoxia. Methods Cultured cardiomyocytes of neonatal rats were used. Hypoxia was imposed to the cardiomyocytes with or without daidzin pretreatment. ROS was measured by C400, apoptosis by the DeadEndSUP>TM/SUP>TUNEL system and MAPK signal pathway by Western blotting. Each group was dealt with more than three times. Results Compared to hypoxia group, that aldehyde dehydrogenase2(ALDH2) specifically inhibited by pretreated daidzin had a high frequency of ROS, apoptosis and an increased activation of MAPK signal enzymes including phosphorylated ERK1/2, JNK and P38Conclusion The cardiac myocytes variation of ROS, apoptosis and MAPK signal path resulting from hypoxia had a close relation with ALDH2 Myocytes showed decreased tolerance to hypoxia after ALDH2 specifically inhibited by daidzin. ALDH2 prevents hypoxia myocardial in

Key words: ALDH2, Hypoxia, Myocyte, Daidzin, MAPK, Western blotting, Rat

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