关键词: 输卵管上皮细胞, 培养时间, 传代, 细胞周期, 流式细胞术, 山羊

Abstract: Objective The experiment was conducted with the objective of determining effects BR> of culture time and subculture on the growth of goat oviductal epithelial cells (OECs). Methods We firstly succeeded in culturing goat OECsEM>in vitro/EM>, and then the cell cycle was examined by flow cytometric analysis and cell apoptosis was determined by Annexin-V and PI staining in goat OECs at different days of confluence and different passages at day 1 of confluence. Results BR>The percentages of （GSUB>0/SUB>/GSUB>1/SUB>） stage, late apoptotic + necrotic and cells with reduced number of polarized mitochondria decreased when OECs were subcultured within Ⅲ passage and confluent less than three days. However, the percentages of S + GSUB>2/SUB>/M stage decreased but late apoptotic + necrotic and cells with reduced number of　polarized mitochondria increased with prolonged culture and passage. Conclusion A better development of cocultured embryos w

Key words: Oviducqatal epithelial cells, Days of confluence, Passage, Cell cycles, Flow cytometry, Goat