›› 2011, Vol. 42 ›› Issue (2): 180-184.doi: 10.3969/j.issn.0529-1356.2011.02.008

• 论著 • 上一篇    下一篇

P>亚硒酸钠抑制人胃癌SGC-7901细胞系增殖和hTERT的表达/P>

苏衍萍*;王慧;葛丽; 刘立伟;魏丽华   

  1. 泰山医学院组织学胚胎学教研室, 山东 泰安271000
  • 收稿日期:2010-04-26 修回日期:2010-05-18 出版日期:2011-04-06
  • 通讯作者: 苏衍萍

Effects of sodium selenite on the growth and hTERT expression of gastric cancer SGC-7901 cell line

  1. Department of Histology and Embryology, Taishan Medical College, Shandong Taian271000,China
  • Received:2010-04-26 Revised:2010-05-18 Online:2011-04-06
  • Contact: SU Yan-ping

关键词: 亚硒酸钠, SGC-7901细胞系, 端粒酶反转录酶, 反转录-聚合酶链反应

Abstract: ObjectiveTo investigate effects of sodium selenite on human gastric cancer SGC-7901 cell growth and hTERT expression and to explore the mechanism. MethodsSGC-7901 cells were treated with different concentrations(0μmol/L,0.5μmol/L,2.5μmol/L,5μmol/L,8μmol/L) of sodium selenite for 24 hours, 48 hours, 72 hours and 96 hours. Growth inhibition of SGC-7901 cells was detected by assay of MTT. Immunohistochemical SABC method and RT-PCR assay were used to study the changes of hTERT proteins and hTERT mRNA in SGC-7901 cells. ResultsSodium selenite significantly suppressed the growth of SGC-7901 cells. The cell growth rate reduced in a dose-and time-dependent manner. The same changes had occurred for the hTERT protein level in SGC-7901 cells. The averae absorbance (AA) of hTERT protein in SGC-7901 cells was gradually lower along time, unto 96 hour it was significantly decreased in 0.5μmol/L sodium selenite group or 2.5μmol/L sodium selenite group than that in blank control group(EM>P/EM><0.01). It was significantly decreased in 5μmol/L, 8μmol/L sodium selenite group than in blank control group all time (EM>P/EM><0.01). RT-PCR results demonstrated there was no obvious alteration of hTERT mRNA levels after 0.5μmol/L sodium selenite treatment. hTERT mRNA levels were gradually reduced along time in 2.5μmol/L sodium selenite group, there was significantly different after 48 hours treatment in 2.5μmol/L sodium selenite group than in blank control group. hTERT mRNA levels were significantly decreased all time in 5μmol/L, 8μmol/L sodium selenite group than in blank

Key words: Sodium selenite, SGC-7901 cell, Telomerase reverse transcriptase, RT-PCR

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