关键词: 半枝莲总黄酮, Bel-7402, 磷脂转运蛋白, 基因重组, 反转录-聚合酶链反应, 免疫印迹法, 免疫组织化学

Abstract: Objective To construct recombinant phospholipid transfer protein(PLTP) cell model and evaluate the effect mechanisms of Barbata flavonoids(BF),through the test of their suppressive function of PLTP in Bel-7402 cells. MethodsPLTP cDNA flag was amplified with reverse transcription-polymerase chain reaction(RT-PCR), PLTP/p3XFLAG-MV-14 was constructed and transfected into Bel-7402 cells. The Bel-7402 cells influenced by Barbata flavonoids were analyzed by immunohistochemical assay. ResultThe expression of PLTP was decreased in Bel-7402 cells treated with 160 mg/L Barbata flavonoids after 24hours,48hours,72hours as compared with the controls（EM>Ｐ/EM><0.05, EM>Ｐ/EM><0.05,EM>Ｐ/EM><0.05）.Under high, middle, low-dose BF, treated after 24hours,48hours,72hours, the expression of PLTP had no significant difference as compared with model group（EM>P/EM>>0.05）. ConclusionWe used gene recombination technology to build recombinant gene cell Bel-7402

Key words: Barbata flavonoids, Bel-7402, Phospholipid transfer protein, Recombinant Gene, RT-PCR, Western blotting, Immunohistochemisitry