Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn

doi:10.16098/j.issn.0529-1356.2015.01.004

Abstract

Abstract:

Objective To explore the effect of panax notoginseng saponins (PNS) pretreatment on the expression of glutamine synthetase (GS) in the inflammatory visceral pain in the rat spinal dorsal horn. Methods Twenty-seven SD rats were randomly divided into normal group, PNS pretreatment group, and vehicle control group. Fifteen days after pretreatment, the model of inflammatory visceral pain was made by intraperitoneal injection of 1% acetic. The visceral pain index of experimental rat was record every 15 minutes. The small intestine tissue was stained with HE. The thoracic spinal cord slices were immunostained with anti-glutamine-synthetase antibody, then observed and compared under an optical microscope. Results The score result of the vehicle control group was significantly higher than that of the PNS pretreatment group. Compared with the normal group, the small intestine tissues of the two experimental groups showed significantly inflammatory response with thickness submucosa, but there was no significantly difference among experimental comparison groups. Compared with the normal group, the GS expression of the two experimental groups was reduced apparently, but the group of PNS pretreatment was significantly higher than the vehicle control group. Conclusion PNS pretreatment may affect the acid-induced inflammatory visceral pain via promoting the experience of GS in the spinal dorsal horn.

Key words: Inflammatory visceral pain, Panax notoginseng saponins, Glutamine synthetase, Visceral pain index, Immunohistochemistry, Rat

ZHANG Ren-li WAN Wei LIU Hong ZENG Jie PENG Tian-hong LIU Zheng-hai*. Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn[J]. AAS, 2015, 46(1): 20-24.

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