Acta Anatomica Sinica

AAS ›› 2015, Vol. 46 ›› Issue (1): 118-121.doi: 10.16098/j.issn.0529-1356.2015.01.021

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Exploring the method of preparing brain slices for patch clamp recording

CHEN Xing1 LIU Xiao-dong2 LI Lin3 WANG Ke4 BAI Wen-pei 1* QIN Li-hua4 YU Long-chuan 2*   

  1. 1.Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034,China; 2. Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences,Peking University,Beijing 100871,China 3. Department of Central Laboratory, 
    Peking University First Hospital, Beijing 100034,China 4. Department of Anatomy and Embryology, Peking University Health Science Center, Beijing 100191,China
  • Received:2014-07-01 Revised:2014-08-10 Online:2015-02-06 Published:2015-02-06
  • Contact: Bai Wen-pei ,YU Long-chuan E-mail:bwp66@163.com

Abstract:

  Objective To explore a method of preparing brain slices for patch clamp technique and to analyze effects of age,solutions and processes of operation on neurons of brain slices. Methods Sixty Sprague-Dawley rats were anesthetized and quickly decapitated. Their brains were cooled and dissected then 300μm coronary brain slices were cut and incubated in the artifical cerebrospinal fluid at 33℃ for 45 minutes. Neurons were observed under an infrared differential aberration interference microscope. Results Neurons showed clear boundary, and three-dimensional appearance and nuclei and particles were not seen. The activity of brain slices depended on various experiment conditions.Conclusion The activity of neurons in the brain slice is associated with age,solutions and processes of operation, thus in experiments we should strictly control these factors.

Key words: Brain slice, Neuron, Patch clamp, Rat