Effects of juglone on the proliferation and apoptosis of HeLa cells

doi:10.16098/j.issn.0529-1356.2015.05.011

Abstract

Abstract:

[Abstract]：AIM:To Objective To explore the pro-apoptotic mechanism of juglone in human cervical cancer HeLa cells, and to study its associated signal transduction pathways. Methods Cultured HeLa cells were incubated with 30μmol/L juglone for 24,48 and 72 hours. The proliferation of HeLa cells was detected by methyl thiazolyl tetrazolium (MTT)assay. The cell apoptosis was detected by Hoechst 33258 staining and flow cytometry (FCM); The expressions of Bcl-2，Bax and Caspase-3,Akt,pAkt and PTEN were detected by Western blotting. Results MTT results showed that the HeLa cell growth was greatly inhibited in a time dependent manner (P<0.05,P<0.01) when compared with the control group. After treatment on HeLa cells for 4，8 and 12 hours, typical morphological changes of cell apoptosis such as karyopyknotic pyknic hyperfluorescence bolus，nuclear fragmentation and apoptotic body were observed by Hoechst 33258 staining. The early apoptosis ratio was increased significantly when compared with the control group. Western blotting results showed that the expression of Bcl-2 and pAkt decreased obviously，whereas those of Bax, Caspase-3 and Akt increased significantly in the juglone treatment group when compared with the control group. Conclusion Juglone may inhibit PI3K/Akt pathway, thereby promoting HeLa cells apoptosis.

Key words: Juglone, PI3K/Akt, HeLa cell, MTT, Hoechst 33258 staining, Western blotting

ZHANG Wei ZHANG Ruo-wen ZHU Wen-he LI Yan LUO Jun ZHAO Xing-yu XU Jun-jie JIANG Yan-xia Lü Shi-Jie*. Effects of juglone on the proliferation and apoptosis of HeLa cells[J]. AAS, 2015, 46(5): 641-645.

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