Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro

doi:10.16098/j.issn.0529-1356.2015.06.012

Abstract

Abstract:

Objectiv To study the radiosensitivity of dihydromyricetin (DMY) on human non-small cell lung cancer A549 cells, and to explore a new drug for lung cancer radiotherapy sensitization.Methods MTT assay and colony formation assay were used to determined the IC₂₀ of dihydromyricetin at different density A549 cells. Flow cytometry (FCM) was used to detect the change of A549 cell cycle phase impacted by DMY of IC₂₀during different time (6, 12, 24, 48hours). Colony formation assay was used to determine the radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro. Results The proliferation of A549 cells was inhibited in a dose-dependent manner, MTT assay showed the IC20 was 40mg/L and colony formation assay showed the IC₂₀was 7mg/L. FCM cycle experiment showed that the rate of G2/M phase ratio obviously increased when A549 cells was incubated 24 hours with DMY；A significant sensibilization leading by the effect of radiotherapy sensibilization on A549 cells after DMY pre-intervention 24 hours was not detected by clone formation sensibilization assay.Conclusion The proliferation of A549 cells was inhibited in a dose-dependent manner of dihydromyricetin, and the effect was influenced by cell density. Although the rate of G₂/M phase ratio obviously increased when A549 cells were incubated 24 hours with DMY, there was no significant sensibilization on A549 cells after DMY pre-intervention 24 hours. A further study should be made.

Key words: Dihydromyricetin, A549 cell, Radiosensitivity, Sensibilization, Flow cytometry, Human

SUN Da-yong BI Hong-dong LIANG Hong-hu ZUO Yan-zhen*WANG Ai-min JIAO Hai-tao. Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro[J]. AAS, 2015, 46(6): 797-801.

References

［1］Zhao WY, Sun XCh. Progression of tumor radiosensitization［J］. Chinese Clinical Oncology, 2012, (7): 655-659. (in Chinese)
赵维勇, 孙新臣. 放射治疗增敏的研究现状［J］. 临床肿瘤学杂志, 2012, (7): 655-659.
［2］Cen JH. Study on apoptosis-inducing mechanism of dihydromyricetin on human liver cancer HepG2［J］. The Journal of Practical Medicine, 2014, (11): 1712-1715. (in Chinese)岑钧华. 二氢杨梅素诱导肝癌细胞株HepG2凋亡途径的探讨［J］. 实用医学杂志, 2014, (11): 1712-1715.
［3］Hou XL, Wang WQ, Shi ChY, el al. Research progress in pharmacological effects of dihydromyricelin［J］. Chinese Traditional and Herbal Drugs, 2015, 46(4): 603-609. (in Chinese)
侯小龙, 王文清, 施春阳,等. 二氢杨梅素药理作用研究进展［J］. 中草药, 2015, 46(4): 603-609.
［4］Yu CC, Hung SK, Liao HF, et al. RAD001 enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/M checkpoint［J］. Anticancer Res, 2014,34(6): 2927-2935.
［5］Zhou FZh, Zhang XY, Guo Y. Research progress on antitumor effect and mechanisms of dihydromyricetin［J］. Chinese Archives of Traditional Chinese Medicine, 2010, 28(9):1914-1916. (in Chinese)
周防震, 张晓元, 郭勇. 二氢杨梅素抗肿瘤活性及其机制的研究进展［J］. 中华中医药学刊, 2010,28(9):1914-1916.
［6］Zhou FZh, Zhang XY, Sun FY, et al. Anti proliferation effect of dihydromyricetin on MDA-MB-231 cell in vitro［J］. Cancer Research on Prevention and Treatment, 2012, 39(1): 95-97. (in Chinese)
周防震, 张晓元, 孙奋勇, 等. 二氢杨梅素对人乳腺癌细胞MDA-MB-231的体外抗增殖作用［J］. 肿瘤防治研究, 2012, 39(1): 95-97.
［7］Franken NA, Rodermond HM, Stap J, et al. Clonogenic assay of cells in vitro［J］. Nat Protoc, 2006,1(5):2315-2319.
［8］Yuan ZhY, Liu XB, Zhuang HQ, et al. Radiosensitizing effects of h-R3 with different time series of administration on human lung cancer cells expressing various EGFR levels［J］. Chinese Journal of Clinical Oncology, 2011,(17):1019-1023. (in Chinese)
袁智勇, 刘晓斌, 庄洪卿, 等. 尼妥珠单抗不同给药时序对人肺癌细胞系的放射增敏作用［J］. 中国肿瘤临床, 2011,(17):1019-1023.
［9］Zhang XY, Wu H, Lin X, et al. Scavenging effect of dihydromyricetin on the free radicals by ESR ［J］. Modern Food Science and Technology, 2010,26(10):1040-1042,1070.
张晓元, 吴晖, 林霞, 等. 用ESR检测二氢杨梅素对自由基的清除作用的研究［J］. 现代食品科技, 2010,26(10):1040-1042,1070.
［10］Gu XZh, Yin WB, Yu ZH, et al. Radiation Oncology［M］. 4th ed. Peking: China Union Medical College Press, 2007: 227-228. (in Chinese)
谷铣之, 殷蔚伯, 余子豪, 等. 肿瘤放射治疗学［M］. 第4版, 北京: 中国协和医科大学出版社, 2007: 227-228.
［11］Song MJ, Liu H, Shen YL. The research progress of flavonoids radiosensitization agents［J］. Chinese Journal of Hospital Pharmacy, 2013, (12): 997-999, 1002. (in Chinese)宋梦姣, 刘宏, 申玉莉. 黄酮类放射增敏剂的研究进展［J］. 中国医院药学杂志, 2013, (12): 997-999, 1002.
［12］Hermann RM, Fest J, Christiansen H, et al. Radiosensitization dependent on p53 function in bronchial carcinoma cells by the isoflavone genistein and estradiol in vitro［J］. Strahlenther Onkol, 2007,183(4):195-202.

[1]	LIU Xiao-li XU Lin WU Ai-xue WEN Cai-yun LI Ru-hua WU An-ting CHEN Dai-qian CHEN Cheng-chun. Analysis of cerebral gray matter structure in multiple sclerosis and neuromyelitis optica [J]. Acta Anatomica Sinica, 2024, 55(1): 17-24.
[2]	BAI He TENG Ye FENG Lei MENG Hai-wei1 TANG Yu-chun LIU Shu-wei. 3D hippocampal segmentation based on spatial and frequency domain features adaptive fusion and inter-class boundary region enhancement [J]. Acta Anatomica Sinica, 2024, 55(1): 73-81.
[3]	YIN Shi-qin YANG Si-yi WANG Rui-han YOU Gui-xuan YANG Ying-qiu ZHANG Lei. Distal tibiofibular syndesmosis fibular notch typing and its clinical significance based on CT [J]. Acta Anatomica Sinica, 2024, 55(1): 82-87.
[4]	YANG Yang SHI Jun LI Kun MA Yuan ZHANG Shao-jie HOU Er-fei WANG Chao-qun CHEN Jie WANG Xing LI Zhi-jun. Finite element analysis of cervical intervertebral discs after removing different ranges of uncinate processes [J]. Acta Anatomica Sinica, 2024, 55(1): 88-97.
[5]	SUN Lei WANG Xing-yu XIE Shui-hua. Risk analysis of re-fracture after percutaneous kyphoplasty in elderly patients with osteoporotic thoracolumbar compression fractures and construction of acolumnar graph prediction model#br# [J]. Acta Anatomica Sinica, 2024, 55(1): 98-104.
[6]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[7]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[8]	HAN Yi CAI Li FENG Xiao . Risk analysis of bone cement leakage after percutaneous puncture vertebroplasty for osteoporotic spinal compression fractures and construction of a predictive model with column line drawings [J]. Acta Anatomica Sinica, 2023, 54(6): 710-715.
[9]	HE Ke-ke LI Yuan-di WEN Ting-hao ZHU Jie GAO Jie HU Rong HAN Feng SU Min . Erythrocyte membrane-associated protein inhibiting Th17 cell differentiation via IL-6/STAT3/ROR-γt signaling pathway in experimental autoimmune encephalomyelitis mice encephalomyelitis mice [J]. Acta Anatomica Sinica, 2023, 54(5): 538-545.
[10]	DU Da-peng SUN Yu ZHANG Min WANG Han-qin . Shear stress regulateing the phosphorylation of endothelial nitric oxide synthase Ser633 and Ser1177 in human umbilical vein endothelial cells through Pim1/Akt pathway [J]. Acta Anatomica Sinica, 2023, 54(5): 546-552.
[11]	ZHOU Chun-hui XIE Sheng-qiang WANG Jiang-ting LAN Xiao-juan ZHANG Jian-ning ZHAO Hu-lin . Application of multimodal electromagnetic navigation in endoscopic endonasal skull base anatomical measurement of fresh cadavers [J]. Acta Anatomica Sinica, 2023, 54(5): 560-566.
[12]	LIU Yan-jing SHI Yong-hong . Automatic extraction of point cloud on cartilage surface of intraoperative knee using FPFH-PointNet [J]. Acta Anatomica Sinica, 2023, 54(5): 553-559.
[13]	MI Si-rong LIU Guang-xing ZHANG Zhen-wu YU Peng-hui JU Xiao-jun RAO Li-bing LI Li . Accuracy of 3D printing skull under different CT layer thickness [J]. Acta Anatomica Sinica, 2023, 54(5): 575-581.
[14]	ZHU Qiu-liang YU Xiang-ping MA Jun CHEN Yun-yun LIN Fang RUAN Wen-bin . Adjustment of X-ray angle intraoperation based on the anatomic shape of femoral neck section [J]. Acta Anatomica Sinica, 2023, 54(5): 586-592.
[15]	LI Xiao-jun ZHANG Ya-min. Screening ferroptosis related genes influencing prognosis of colon cancer through bioinformatics analysis [J]. Acta Anatomica Sinica, 2023, 54(4): 445-452.