Correlation between the expression of P34H and the activity of hyaluronidase in human spermatozoa

doi:10.16098/j.issn.0529-1356.2017.02.013

Abstract

Abstract:

Objective To investigate the correlation between the level of P34H expression and the activity of hyaluronidase in human spermatozoa. Methods Eighty eight semen samples were collected, 68 cases were in the infertile group, and 20 in the normal control group. Semen routine analysis was referred to the WHO standard method. According to the difference of semen parameters, 68 cases of infertile males were divided into the infertile group with normal semen parameters and the abnormal semen parameters. Western blotting was used to detect the level of P34H expression on spermatozoa. The P34H-positive rate on human spermatozoa was determined by indirect immunofluorescent staining using anti-P34H antibody. The HYD-positive rate and HYD-activity intensity in all samples were examined by improved fixed-substrate film method. Results The level of P34H protein expression and the percentage of the P34H-positive rate in infertile groups with normal semen parameters and abnormal semen parameters were significantly lower than that in the control group (P<0.05). The activity of HYD (HYD-positive rate, HYD-activity intensity) in the infertile groups with normal semen parameters and abnormal semen parameters were also significantly lower than that in control group (P<0.05). The relation between the P34H protein expression and HYD-positive rate, HYD-activity intensity had a significant positive correlation (r=0.449, 0.431；P<0.01); the relation between the the percentage of P34H-positive rate and HYD-positive rate, HYD-activity intensity had a significant positive correlation (r=0.727, 0.691；P<0.01). Conclusion The level of P34H protein expression and the percentage of the P34H-positive rate are decreased, while the activity of HYD (HYD-positive rate, HYD-activity intensity) is reduced in male infertility.

Key words: Sperm, P34H, Hyaluronidase, Male infertility, Western blotting, Immunofluorescence, Human

MA Xiao-ping GAO Xiao-qin YUE Ying-quan WANG Jun. Correlation between the expression of P34H and the activity of hyaluronidase in human spermatozoa[J]. Acta Anatomica Sinica, 2017, 48(2): 193-198.

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