Compatibility between adipose derived stem cells of rats and three dimensional printing gelatin scaffold

doi:10.16098/j.issn.0529-1356.2017.02.016

Abstract

Abstract:

Objective To observie the compatibility of rat adiposederived stem cells (ADSCs) and three dimensional printing (3DP) gelatin scaffolds crosslinked with glutaraldehyde, the most suitable for cell growth aperture, in order to provide experimental basis for constructing tissue-engineered tissues or organs. Methods The enzyme digestion method was adopted to separate and extract rat ADSCs. Flow cytometry and multidirectional differentiation method were used for identification. The ADSCs were cultured with 3DP gelatin scaffolds of different aperturs. Scanning electron microscopy (SEM) and transmission electron microscope (TEM) were used to observe the ultra-microstructure. The cell vitality analyzer was used to detect its survival rate. MTT method was used to detect the effects of two dimensional (2D) and three dimensional (3D) cultural methods on ADSCs cell vitality. Results The seeding ADSCs had multi-directional differentiation potential, and had the basic characteristics of stem cells. After inoculation of ADSCs to 3DP gelatin scaffolds, cells were oval or spindle observed by SEM, which was different from the traditional 2D culture form. The cells scattered within the scaffold gap, the structures such as the nucleus and organelles were clear, which demonstrated a good compatibility with scaffold. The cell survival rate of 90μm aperture scaffold was the highest, and 3D cultural method was more advantageous to maintain the vitality of ADSCs. Conclusion The compatibility of ADSCs with 3DP gelatin scaffold is good, and 90μm aperture scaffold is most suitable for the growth of ADSCs.

Key words: Adipose-derived stem cells, Three dimensional printing gelatin scaffold, Compatibility, Electron microscope, Flow cytometry, Rat

TIAN Xiao-hong ZHANG Bin FANG Yan BAI Shu-ling AO Qiang TONG Hao. Compatibility between adipose derived stem cells of rats and three dimensional printing gelatin scaffold[J]. Acta Anatomica Sinica, 2017, 48(2): 209-216.

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