Association between G894T polymorphism of endothelial nitric oxide gene and pregnancyinduced hypertension syndrome in Han group of Qinghai

doi:10.16098/j.issn.0529-1356.2017.03.018

Abstract

Abstract:

Objective To investigate the association between the gene polymorphism of endothelial nitric oxide synthase (eNOS) and pregnancy-induced hypertension syndrome (PIH) in Han population of Qing hai. Methods eNOS genotype with polymerase chain reaction-restriction fragment length polymorphism was performed in 138 PIH subjects and 135 normal pregnancy subjects. The mutation was confirmed by DNA sequencing. Results The frequencies of eNOS GG, GT and TT genotypes were 17.4 %, 82.6 %,0 in PIH group and 0 %, 95.6 %,4.4 % in control Group.There were differences between the two groups of genotype frequency distribution (P<0.05). The frequencies of the two alleles G and T were 58.7 %, 41.3 % in PIH group and 47.8 %, 52.2 % in control group,respectively(P<0.05).The frequency of G gene in PIH group was higher than the control group. Conclusion The study suggests an association between the G894T polymorphism of eNOS gene and PIH in Han group of Qinghai.G allele may be a susceptible gene to PIH（OR=1.229,95%CI: 1.048～1.441）and T allele may be a protective gene to PIH. Pregnant women with GG genotype may be a high susceptible group of pregnancy induced hypertension in Qinghai province.

Key words:

Pregnancy-induced hypertension syndrom, Polymorphism, Endothelial nitric oxide synthase, Restriction fragment length polymorphism polymerase chain reaction, Pregnant woman

LI Hong-rong LI Changp-xing WANG Xiang-lin ZHUANG Wen-ting YAN Shuai LI Jian-hua. Association between G894T polymorphism of endothelial nitric oxide gene and pregnancyinduced hypertension syndrome in Han group of Qinghai[J]. Acta Anatomica Sinica, 2017, 48(3): 353-357.

References

［1］Cao ZY. Chinese Obstetrics and Gynecology ［M］. 3th ed. Beijing: People’s Medical Publishing House, 2014 : 503-521.(in Chinese)

曹泽毅.中华妇产科学［M］.第3版,上册.北京:人民卫生出版社,2014 : 503-521.

［2］Francisco JV, Alejandra PS, Maria JT, et al. Pathogenesis of preeclampsia: the genetic component ［J］. J Pregnancy, 2012, 2012: 632732.

［3］Wang J, Du B, Shan AJ, et al. A single endothelial nitric oxide synthase of gene polymorphism on nitric oxide levels ［J］. Medical Review,2014, (04) : 580-583. (in Chinese)

王进,杜波,单爱军,等.内皮型一氧化氮合酶基因多态性对一氧化氮水平的影响［J］. 医学综述,2014,04 : 580-583.

［4］Molana A, Mehrpour M, Vousooghi N, et al. Effect of NOS3 gene polymorphism on response to Tricyclic antidepressants in migraine attacks ［J］. Iran J Neurol, 2014, 13 (3) :146-151.

［5］Kuo TY, Kang MJ, Chen JW, et al. A two-stage matched case-control study on multiple hypertensive candidate genes in Han Chinese ［J］. Am J Hypertens, 2012, 25 (7) : 804-811.［6］Lu M, Huang ShP, Zheng LM, et al. Preeclampsia patients the expression of nitric oxide synthase and its endogenous inhibitor ADMA and significance ［J］. Journal of Maternal and Child Health Care in China, 2016, 31(10) : 2203-2206. (in Chinese)

卢敏,黄珊萍,郑林媚,等. 子痫前期患者一氧化氮合酶及其内源性抑制物ADMA的表达及意义［J］.中国妇幼保健, 2016, 31(10) : 2203-2206.

［7］Dai B, Liu T, Zhang B, et al. The polymorphism for endothelial nitric oxide synthase gene, the level of nitric oxide and the risk for preeclampsia: a meta-analysis ［J］. Gene, 2013, 519 (1) : 87-93.

［8］Sharma D, Hussain SA, Akhter N, et al. Endothelial nitric oxide synthase (eNOS) gene Glu298Asp polymorphism and expression in North Indian preeclamptic women ［J］. Pregnancy Hypertension An International Journal of Womens Cardiovascular Health, 2014, 4(1) : 65.

［9］Wang XH, Xu XM. Nitric oxide synthase, nitric oxide and pregnancy ［J］. Modern Obstetrics and Gynecology of Pregnancy 2012, (7) : 568-570. (in Chinese)

王霞红,徐先明.一氧化氮合成酶,一氧化氮与妊娠［J］. 现代妇产科进展, 2012, (7):568-570.

［10］Rafikov R, Fonseca FV, Kumar S, et al. eNOS activation and NO function:Structural motifs responsible for the post translational control of endothelial nitric oxide synthase activity ［J］. J Endocrinol, 2011, 210 (3) : 271-284.

［11］Shi Y, Zhang Y, Shao J, et al. Nitric oxide of astrocyte axon growth factor 1 expression and the influence of the migration ［J］. Acta Anatomica Sinica, 2013, 44(5) : 635-640. (in Chinese)

施月,张晔,邵杰,等. 一氧化氮对星形胶质细胞轴突生长因子-1表达及迁移的影响［J］. 解剖学报, 2013,44 (5) : 635-640.

［12］Xie LJ, He D, Hong FF, et al. Nitric oxide and vascular aging［J］. Journal of Nanchang University (Medical Edition), 2014, (03) : 70-75，91. (in Chinese)

谢林均,何迪,洪芬芳, 等. 一氧化氮与血管老化［J］. 南昌大学学报(医学版), 2014, (03): 70-75，91.

［13］Li Q, Yin ZhR, Cai H, et al. The effects of nitric oxide synthase decoupling linked the role of hypertension ［J］. Chinese Journal of Hypertension, 2013, (12) : 1105-1109. (in Chinese)

李强, 尹志润, 才华, 等. 内皮型一氧化氮合酶解耦联在高血压发生中的作用［J］. 中华高血压杂志, 2013, (12) : 1105-1109.

［14］Fang Ch, Zeng H, Zhou HH, et al. Research progress on the relationship between nitric oxide synthase and angiotensin converting enzyme gene polymorphism and coronary heart disease ［J］. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, (2): 222-228. (in Chinese)

房超,曾慧,周宏灏,等.一氧化氮合酶和血管紧张素转换酶基因多态性与冠心病相关性研究进展［J］. 中国临床药理学与治疗学,2013,(2):222-228.

［15］Mcdonald DM, Alp NJ, Channon KM, et al. Functional comparison of the endothelial nitric oxide synthase Glu298Asp polymorphic variants in human endothelialcells ［J］. Pharmacoqenetics, 2004, 14(12):831-839.

［16］Lu HX, Wang YX, Chen Y, et al. Association between endothelial nitric oxide synthase (ENOS) G894T polymorphism and high altitude (HA) adaptation:a meta-analysis ［J］. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 2015,31(6):517-523.

［17］Bian ShZh, Huang L. Roles of endogenous nitric oxide in acute mountain sickness ［J］.Chinese Journal of Pathophysiology, 2013, (1) : 174-178. (in Chinese)

卞士柱,黄岚. 内源性一氧化氮在急性高原病中的研究进展［J］. 中国病理生理杂志,2013, (1):174-178.

［18］Zhao ZhP, Shi XG, Kang LL, et al. The role of adaptation of nitric oxide in high altitude ［J］. Foreign Medical (Medical Geography), 2013,34 (3): 206-210. (in Chinese)

赵志鹏, 史旭刚, 康龙丽, 等. 一氧化氮在高海拔适应中的作用［J］. 国外医学(医学地理分册), 2013, 34(3):206-210.

［19］Ma Q, Lü J, Huang K, et al. Endothelial nitric oxide synthase gene G894T polymorphism and risk assessment for pregnancy-induced hypertension: evidence from 11700 subjects ［J］. Hypertens Res, 2016, 39 (12) : 899-906.

［20］Alpoim PN, Gomes KB, Pinheiro Mde B, et al. Polymorphisms in endothelial nitric oxide synthase gene in early and late severe preeclampsia ［J］. Nitric Oxide, 2014, (42): 19-23.［21］Sakar MN, Atay AE, Demir S, et al. Association of endothelial nitric oxide synthase gene G894T polymorphism and serum nitric oxide levels in patients with preeclampsia and gestational hypertension ［J］. Matern Fetal Neonatal Med, 2015, 28(16) :1907-1911.

［22］Rahimi Z, Aqhaei A, RahimiZ, et al. Endothelial nitric oxide synthase (eNOS) 4a/b and G894T polymorphisms and susceptibility to preeclampsia ［J］. Repord infertil, 2013,14(4):184-189.

［23］Cai X, Zhao J, Jiao YH, et al. The relationship between the polymorphism of nitric oxide synthase gene and pregnancy induced hypertension syndrome ［J］. Chinese Journal of Perinatal Medicine, 2005, (2):12-15. (in Chinese)

蔡霞,赵静,焦永慧,等.一氧化氮合酶基因多态性与妊娠高血压综合征的关系［J］. 中华围产医学杂志, 2005, (2) :12-15.

［24］Ding YSh, Guan lX, Wang YH, et al. MTHFR gene and eNOS gene polymorphism and the pathogenesis of pregnancy-induced hypertension syndrome association study ［J］. Chinese Journal of Healthy Birth and Heredity, 2008, 16(7) : 12-14. (in Chinese)

丁砚生, 管立学, 王育慧, 等. MTHFR基因和eNOS基因多态性与妊娠高血压综合征发病的相关性研究［J］. 中国优生与遗传杂志, 2008, 16(7) : 12-14.

［25］Zhou M, Dang ShY, Wang JF, et al. Meta analysis of the relationship between eNOS gene G894T polymorphism and essential hypertension in Chinese Han population ［J］. Chinese Journal of Evidence Based Cardiovascular Medicine, 2012, 4(5) : 408-411. (in Chinese)

周明, 党书毅, 王俊峰, 等. 中国汉族人群eNOS基因G894T多态性与原发性高血压关系的Meta分析［J］. 中国循证心血管医学杂志, 2012, 4(5) : 408-411.

［26］Srivastava K, Narang R, Sreenivas V, et al. Association of eNOS Glu298Asp gene polymorphism with essential hypertension in Asian Indians ［J］.Clin Chim Acta, 2008, 387(1-2):80-83.

［27］Wang XH, Ni RZh. The Association between G894T polymorphism of eNOS gene and essential hypertension in Han group of Yunnan ［J］. Journal of Kunming Medical University, 2010, 31(7) : 72-75. (in Chinese)

王晓焕,倪锐志. eNOS基因G894T多态与云南省汉族高血压相关性［J］. 昆明医学院学报, 2010,31 (7) : 72-75.

[1]	DU Da-peng SUN Yu ZHANG Min WANG Han-qin . Shear stress regulateing the phosphorylation of endothelial nitric oxide synthase Ser633 and Ser1177 in human umbilical vein endothelial cells through Pim1/Akt pathway [J]. Acta Anatomica Sinica, 2023, 54(5): 546-552.
[2]	LUO Yan-ping LIU Chao GU Xi-xi CHEN Jian-ming LAN Yan WEI Ye-sheng. Distribution characteristics of polymorphisms of microRNA-30 gene in Guangxi Zhuang population and their association with serum lipid levels [J]. Acta Anatomica Sinica, 2023, 54(4): 400-404.
[3]	WU Zhen DUAN Qian YUAN Ming WANG Xue-ni CHEN Wen-jing LI Yu-qin E-Luo-Ji LI Jian-hua. Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province [J]. Acta Anatomica Sinica, 2023, 54(3): 313-318.
[4]	LI Fan MA Zhan-bing ZHANG Jing YANG Meng-yi WANG Yuan MA Qian LU Hong HUO Zheng-hao DANG Jie . Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio [J]. Acta Anatomica Sinica, 2023, 54(2): 208-215.
[5]	DUAN Qian LI Jian-hua LI Wen-jia WANG Ru WU Zhen YUAN Ming. Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality [J]. Acta Anatomica Sinica, 2023, 54(1): 63-69.
[6]	LI Jing-yun LU Yun-hua ZHENG Li-xia ZHAO Gang LIU Hui-li. Relationship between the G894T polymorphism of endothelial nitric oxide synthase gene and lipid metabolism among hypertensive disorder complicating pregnancy patients [J]. Acta Anatomica Sinica, 2022, 53(6): 737-743.
[7]	YUAN Ming DUAN Qian LI Wen-jia WANG Ru WU Zhen LI Jian-hua. Polymorphism of 5,10-methylenetetrahydrofolate reductase gene rs1801131 in Qinghai Han patients with hypertensive disorders complicating pregnancy [J]. Acta Anatomica Sinica, 2022, 53(5): 594-599.
[8]	LEI Ming TAN Tan HUANG Yan-yun WEI Ye-sheng. Association between the polymorphism and expression level of the family microRNA-181 and susceptibility of ischemic stroke [J]. Acta Anatomica Sinica, 2022, 53(4): 470-478.
[9]	DUAN Qian LI Jian-hua LI Wen-jia WANG Ru. Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province [J]. Acta Anatomica Sinica, 2022, 53(3): 354-359.
[10]	GU Xi-xi ZENG Zhi-neng HE Yong-ling SHI Xiang WEI Ye-sheng. Distribution of hypoxia inducible factor 3A gene rs11672731 and rs2072491 single nucleotide polymorphisms in Guangxi Han population [J]. Acta Anatomica Sinica, 2021, 52(4): 647-651.
[11]	LI Wen-jia WANG Ru DUAN Qian LI Chang-xing LI Hong-rong LI Jian-hua. Association between the polymorphism of aldosterone synthase gene promoter-344T/C and preeclampsia in Qinghai Province [J]. Acta Anatomica Sinica, 2021, 52(2): 306-310.
[12]	HUANG Yan-yun HUANG Hua-tuo LU Yu-lan LEI Ming TAN Tan WEI Ye-sheng. Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels [J]. Acta Anatomica Sinica, 2020, 51(2): 284-288.
[13]	WANG Ru ZHUANG Wen-ting WANG Xiang-lin LI Hong-rong LI Chang-xing KONG De-xia LI Jian-hua. Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai [J]. Acta Anatomica Sinica, 2020, 51(2): 294-299.
[14]	LIU Chun-hong LU Yu-lan WANG Rong HUANG Hua-tuo WEI Ye-sheng. Genetic polymorphism of rs1051169 G/C and rs9984765 T/C in S100B gene in Chinese Guangxi population [J]. Acta Anatomica Sinica, 2019, 50(4): 517-521.
[15]	ZHUANG Wen-ting ZHAO De-xiong WANG Xiang-lin LI Hong-rong LI Chang-xing LI Fu-xin KONG De-xia LI Jian-hua . Serum leptin and polymorphism of leptin receptor gene Gln223Arg of Qinghai patients with pregnancy-induced hypertension syndrome [J]. Acta Anatomica Sinica, 2018, 49(6): 814-818.