Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province

doi:10.16098/j.issn.0529-1356.2022.03.013

Abstract

Abstract:

Objective To investigate the association between polymorphisms of methylene tetrahydrofolate reductase (MTHFR)-677C/T (rs1801133) and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai Province. Methods A total of 139 HDP subjects (HDP group) and 145 normal pregnant subjects (control group) were selected from Qinghai province. The MTHFR-677C/T polymorphism typing in HDP group and control was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The mutation was confirmed by sequencing. Results The frequency of CC CT TT genotype of MTHFR gene in HDP group and control group were 54.68%，35.25%，10.07% and 69.66%，22.06%，8.28% respectively (P<0.05);The frequency of CC genotype (54.68%) in HDP group was lower than that(69.66%) in control group (P<0.05),the frequency of CT genotype ( 35.25%) in HDP group was higher than that (22.06%) in control group (P<0.05),there was no significant difference in the frequency of TT genotype between HDP group and control group (P>0.05);The frequency distribution of alleles C and T of MTHFR-677C/T polymorphisms were different between HDP group and control group (P<0.05),T allele frequency in HDP group was higher than that in control group(χ²=5.568，P<0.05). Conclusion The polymorphisms of MTHFR -677C/T is significantly correlated with hypertensive disorders of pregnancy of Han group in Qinghai Province. The T allele of MTHFR -677C/T polymorphism may be the susceptibility gene of HDP,CT genotype is the susceptibility genotype of HDP.

Key words: Hypertensive disorder of pregnancy, Methylenetetrahydrofolate retractase, Genetic polymorphism, Folate metabolism, Restriction fragment length polymorphism polymerase chain reaction, Pregnant woman

CLC Number:

DUAN Qian LI Jian-hua LI Wen-jia WANG Ru. Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province[J]. Acta Anatomica Sinica, 2022, 53(3): 354-359.

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