Effect of Panax notoginseng total saponin on lipopolysaccharide induced depression-like behavior and expression of microglia in mice

doi:10.16098/j.issn.0529-1356.2018.02.005

Abstract

Abstract:

Objective To investigate the effect of Panax notoginseng total saponins (PNS) on lipopolysaccharide (LPS)-induced depression-like behavior and expression of microglia in mice. Methods Totally 24 male Kunming mice were randomly divided into NS group, LPS group and PNS+LPS group. Depression-like behavior was investigated by novelty-suppressed feeding test, sucrose preference test, tail suspension test and forced swimming test. Expression of microglia marker ionized calcium binding adaptor molecule-1 (IBA-1) in the hippocampus and amygdala was determined by Immunohistochemistry assess. Results LPS led to prolonged latency in the novelty-suppressed feeding test (P<0.05), reduced sucrose preference (P<0.05), and increased immobility time in the tail suspension test (P<0.05) and forced swimming test (P<0.01), while PNS treatment could effectively reverse the depression-like behavior induced by LPS injection (P<0.05). LPS injection led to significantly increased number of IBA1 positive cells in the hippocampus and amygdala (P<0.0001), which was attenuated by PNS treatment (P<0.05).Conclusion Our findings indicate that PNS can alleviate LPS-induced depression-like behavior in mice, which may be attributed to the inhibiting of microglia activation in the hippocampus and amygdala.

Key words: Panax notoginseng total saponins, Lipopolysaccharide, Depression, Inflammatory, Microglia, Immunohistochemistry, Mouse

ZENG Jia-yu WANG Zhen NIU Lei LUO Shi-shi LIU Zheng-hai CAO Wen-yu XU Yang WAN Wei CHEN Xi. Effect of Panax notoginseng total saponin on lipopolysaccharide induced depression-like behavior and expression of microglia in mice[J]. Acta Anatomica Sinica, 2018, 49(2): 166-171.

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