Expression of Golgi phosphoprotein 3 in colorectal cancer tissues and its effects on proliferation, invasion and migration of colorectal cancer cells

doi:10.16098/j.issn.0529-1356.2018.04.008

Abstract

Abstract:

Objective To analyze the expression of Golgi phosphoprotein 3 (GOLPH3) in colorectal cancer tissues and its influence on the proliferation, invasion and migration of colorectal cancer cells. Methods The colorectal cancer tissues and paracancerous tissues were collected from the surgically resected colorectal cancer patients (40 cases) without radiotherapy or chemotherapy before the operation. The expression of GOLPH3 in the colorectal cancer tissues and paracancerous tissues were detected by immunohistochemistry, and the relationship between GOLPH3 and clinicopathological features of colorectal cancer was analyzed. Colorectal cancer cell starin with GOLPH3 stable silent was constructed, and a negative control group and blank control group were established. The expression of GOLPH3 protein of cells in the three groups was detected by Western blotting. MTT assay was used to detect the proliferative activity of cells in the three groups. The invasion ability and migration ability of cells in the three groups were detected by transwell test. Results The expression of GOLPH3 was mainly positive in the cancer tissue sample, and the positive expression rate (65.0%) of the cancer tissues was significantly higher than that of the paracancerous tissues (35.0%) (P<0.05). The GOLPH3 positive expression rate of cancer tissues with TNM stage Ⅲ-Ⅳ, invasion depth≥2 cm, middle-low differentiation and lymphatic metastasis were significantly higher than that of cancer tissues with TNM stageⅠ-Ⅱ, invasion depth<2 cm and high differentiation and without lymphatic metastasis (P<0.05). Compared with the negative control group and the blank control group, the expression level of GOLPH3, the cell proliferation activity, the invasion ability and migration ability rate in the gene silencing group were significantly lower (P<0.05). Conclusion GOLPH3 is highly expressed in the colorectal cancer tissues, and the expression level is related to the tumor staging, invasion depth, differentiation and lymphatic metastasis. Inhibiting the expression of GOLPH3 gene could deregulate the GOLPH3 protein level, and inhibit the proliferation, invasion and migration of colorectal cancer cells.

Key words: Colorectal cancer, Golgi phosphoprotein 3, Gene silencing, Cell proliferation, Immunohistochemistry, Human

LIU Shi-hong WANG Wei MU Xiao-song. Expression of Golgi phosphoprotein 3 in colorectal cancer tissues and its effects on proliferation, invasion and migration of colorectal cancer cells[J]. Acta Anatomica Sinica, 2018, 49(4): 461-468.

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