Expression and age-related changes of centromere A in rat heart

doi:10.16098/j.issn.0529-1356.2018.04.016

Abstract

Abstract:

Objective To study the expression and age-related changes of centromere protein A(CENP-A) in the rat heart. Methods Ten rats in each group were collected from postnatal 1 day, 1 week old, 2 weeks old, 3 weeks old, 4 weeks old, 3 months old, 6 months old. The expression and age-related changes of CENP-A in the rat heart were observed by the immunohistochemistry, immunofluorescence, Western blotting, and Real-time PCR techniques. The CENP-A proteins of seven groups were quantitated by SPSS 20.0 statistical software. Results CENP-A was mainly expressed in the vascular wall, epicardium and epicardial tissue and in a very few cardiomyocytes. The CENP-A in vascular wall was of stable expression with age. But the expression of CENP-A in the epicardium and subepicardial tissues was gradually reduced, which showed that postnatal 1 day was the most and 6 months old are the least. CENP-A in the vascular wall was mainly expressed in the smooth muscle cells, and was basically unchanged with ages.A small number of cardiac myocytes near the subepicardium also express CENP-A, and decreased with age. Conclusion The expression of CENP-A in the rat heart gradually decreased with aging, but in the vascular wall remained stable expression.

Key words: Centromere protein-A, Myocardial tissue, Epicardium, Heart, Immunohistochemistry, Immunofluorescence, Rat

FENG Ying YIN Guo-tian LI Ai-rong GUO Zhi-kun. Expression and age-related changes of centromere A in rat heart[J]. Acta Anatomica Sinica, 2018, 49(4): 512-517.

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