Expression of microRNA-613 in human glioma and its effects on growth and metastasis

doi:10.16098/j.issn.0529-1356.2018.05.003

Abstract

Abstract:

Objective To investigate the expression of microRNA-613(miR-613) in human glioma and its effect on the proliferation and metastasis. Methods Thirty human glioma patients tissues and 30 Non-glioma patients tissueswere detected by Real-time PCR, and to analysie their relationship with clinicopathological characteristics of gliomas. MiR-613mimics and unrelated sequence control(miR-613 con) were transfected into U87 cells using Lipofectamine TM2000. The expression of miR-613 in the two groups of cells was detected by Real-time PCR, the proliferation of cells was detected by CCK8 assay, and the invasion and migration of cells were detected by Transwell assay. Results The expression of miR-613 in Glioma tissues was lower than that in corresponding paracancerous tissues (P<0.05). The expression level of miR-613 in glioma tissue was negatively correlated with pathological grade and positively correlated with survival time (P<0.05). Compared with miR-613 con cells, miR-613 mimics cells proliferation, invasion and migration were significantly decreased (P<0.05). Conclusion MiR-613 can inhibit the proliferation, invasion and migration of glioma cells, and it might be a potential therapeutic target for the treatment of glioma.

Key words: MicroRNA-613, Glioma, U87, Growth, Metastasis, Real-time PCR, Human

LI Chuan-fen JING Wen XIANG Dong-sheng LI Tao JIAO Shu-xin YANG Cui-ling MA Zhi-hong WANG Min. Expression of microRNA-613 in human glioma and its effects on growth and metastasis[J]. Acta Anatomica Sinica, 2018, 49(5): 579-583.

References

［1］ Compilation group: Guidelines for the diagnosis and treatment of gliomas in the central nervous system in China. Guidelines for the diagnosis and treatment of central nervous system gliomas in China (2015)［J］. Chinese Journal of Medical Science, 2016, 96(7): 485-509.（in Chinese）

《中国中枢神经系统胶质瘤诊断和治疗指南》编写组.中国中枢神经系统胶质瘤诊断与治疗指南（2015）［J］.中华医学杂志,2016,96(7):485-509.

［2］ Zhang M, Pan JZh, Liu T, et al. Preliminary study of KCTD10 expression in gliomas［J］. Journal of Hunan Normal University (Medical Sciences), 2016, 13(1):6-8.（in Chinese）张敏,潘剑珍,刘彤,等.KCTD10在神经胶质瘤中表达的初步研究［J］.湖南师范大学学报(医学版),2016,13(1):6-8.

［3］ Zhang P, Feng YN, Wang HZh, et al. Experimental study on the mechanism of Chinese herbal compound antitumor pills in treating glioma［J］. Beijing Journal of Traditional Chinese Medicine, 2017, 36(2): 135-138,193.（in Chinese）

张鹏,冯英楠,王海征,等.中药复方抗瘤丸治疗神经胶质瘤作用机制的实验研究［J］.北京中医药,2017,36(2):135-138,193.

［4］ Zhang YH, Li H, Liu YCh. Role and mechanism of miRNA in tumor radiotherapy sensitivity［J］. Modern Oncology Medicine, 2016, 24(2): 314-318.（in Chinese）

张玉虹,李皓,刘阳晨.miRNA在肿瘤放疗敏感性中的作用及机制［J］.现代肿瘤医学,2016,24(2):314-318.

［5］ Shao J, Kong GM, Shi MY, et al. The role of gastric juice microRNA-133a in diagnosis and prognosis of gastric cancer［J］. Acta Anatomica Sinica, 2017,48(5):550-555.（in Chinese）

邵娟,孔桂美,史明仪,等.胃液microRNA-133a在胃癌诊断和预后评估中的作用［J］.解剖学报,2017,48(5):550-555.

［6］ Lang BJ, Ma JY, Hu YCh, et al. Expression of microRNA-184 in glioma and its prognostic value［J］. Cancer Prevention Research, 2015, 42(5): 48-487.（in Chinese）

郎博娟,马金阳,胡余昌,等.胶质瘤中MicroRNA-184的表达及其预后价值［J］.肿瘤防治研究,2015,42(05):483-487.

［7］ Li XX, Yu Q, Tian Y, et al. Expression and clinical significance of microRNA-221 in human glioma［J］. Chinese Journal of Modern Medicine, 2015, 25(17):26-29.（in Chinese）

李新星,于奇,田羽,等.人脑胶质瘤中microRNA-221的表达及临床意义［J］.中国现代医学杂志,2015,25(17):26-29.

［8］ Xu PX, Li Q, Xu QG, et al. Clinical significance and biological effects of miRNA-210 expression in human glioma［J］. Journal of Hainan Medical College, 2017, 23(12): 1675-1678.（in Chinese）

徐鹏翔,李强,许琼冠,等.miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应的探究［J］.海南医学院学报,2017,23(12):1675-1678.

［9］ Han YCh, Hu ChH, Yang F, et al. The role of miRNA-145 in brain gliomas［J］. Chinese Journal of Clinical Neurosurgery, 2017, 22(7): 486-488, 523.（in Chinese）

韩铖琛,胡晨浩,杨帆,等.miRNA-145在脑胶质瘤中的作用［J］.中国临床神经外科杂志,2017,22(7):486-488,523.

［10］Di G, Wang Z, Wang W, et al.AntagomiR-613 protects neuronal cells from oxygen glucose deprivation/re-oxygenation via increasing SphK2 expression［J］.Biochem Biophys Res Commun, 2017,493(1):188-194.

［11］Zhang X, Zhang H.Diminished miR-613 expression as a novel prognostic biomarker for human ovarian cancer［J］.Eur Rev Med Pharmacol Sci,2016,20(5):837-841.

［12］Guan S, Wang C, Chen X, et al.MiR-613: a novel diagnostic and prognostic biomarker for patients with esophageal squamous cell carcinoma［J］.Tumour Biol,2016,37(4):4383-4391.

［13］Li D, Li DQ, Liu D, et al.MiR-613 induces cell cycle arrest by targeting CDK4 in non-small cell lung cancer［J］.Cell Oncol, 2016, 39 (2):139-147.

［14］Wang W, Zhan H, Wang L, et al.miR-613 inhibits the growth and invasiveness of human hepatocellular carcinoma via targeting DCLK1［J］,Biochem Biophys Res Commun,2016, 473 (4):987-992.

［16］Wu J, Yuan P, Mao Q, et al.miR-613 inhibits proliferation and invasion of breast cancer cell via VEGFA［J］. Biochem Biophys Res Commun,2016,478(1):274-278.

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