Acta Anatomica Sinica

Acta Anatomica Sinica ›› 2018, Vol. 49 ›› Issue (5): 579-583.doi: 10.16098/j.issn.0529-1356.2018.05.003

• Neurobiology • Previous Articles     Next Articles

Expression of microRNA-613 in human glioma and its effects on growth and metastasis

LI Chuan-fen 1,5 JING Wen2 XIANG Dong-sheng3 LI Tao4 JIAO Shu-xin5 YANG Cui-ling5 MA Zhi-hong5 WANG Min 1*   

  1. 1. College of Life Sciences,  Shandong Normal University, Ji’nan 250014, China; 2. College of Physical Education,  Shandong Normal University, Ji’nan 250014, China; 3. Electrical Department, Shandong Vocational College, Ji’nan 250104, China;  4. Department of Microbiology Laboratory, Linyi Disease Prevention and Control Center, Shandong Linyi 276000, China; 5. Department of Neurology, General Hospital of Ji’nan Military Region, Ji’nan 250031, China
  • Received:2018-03-22 Revised:2018-04-04 Online:2018-10-06 Published:2018-10-06
  • Contact: WANG Min E-mail:lcf23598@163.com

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Abstract:

Objective To investigate the expression of microRNA-613(miR-613) in human glioma and its effect on the proliferation and metastasis. Methods Thirty human glioma patients tissues and 30 Non-glioma patients tissueswere detected by Real-time PCR, and to analysie their relationship with clinicopathological characteristics of gliomas. MiR-613mimics and unrelated sequence control(miR-613 con) were transfected into U87 cells using Lipofectamine TM2000. The expression of miR-613 in the two groups of cells was detected by Real-time PCR, the proliferation of cells was detected by CCK8 assay, and the invasion and migration of cells were detected by Transwell assay. Results The expression of miR-613 in Glioma tissues was lower than that in corresponding paracancerous tissues (P<0.05). The expression level of miR-613 in glioma tissue was negatively correlated with pathological grade and positively correlated with survival time (P<0.05). Compared with miR-613 con cells, miR-613 mimics cells proliferation, invasion and migration were significantly decreased (P<0.05). Conclusion MiR-613 can inhibit the proliferation, invasion and migration of glioma cells, and it might be a potential therapeutic target for the treatment of glioma.

Key words: MicroRNA-613, Glioma, U87, Growth, Metastasis, Real-time PCR, Human