Effects of heroin on the renal tissue structures and functions of mice

doi:10.16098/j.issn.0529-1356.2018.06.011

Abstract

Abstract:

Objective To study the poisonous effects of heroin on the kidney tissues of mice. Methods Sixty mice were randomly divided into control group and heroin groups.The mice in heroin group were intraperitoneally injected with 0.2 ml heroin solution twice a day at incremental concentration doses 0.0571, 0.0667 and 0.0706 g/kg each concentration for 15 days.The mice in control group were injected saline at the same volume as that in heroin group.The body weight and kidney weight were weighted and the contents for catalase(Cr)and uric acid(UA)in plasma and contents for malondialdehyde (MDA)as well as the activities of catalase(CAT)，Na^+-K⁺-ATP and lactate dehydrogenase(LDH)in kidney tissues were respectively detected by colorimetry at day 5, day 10 and day 15 treatments.And the morphological structure of kidneys were observed under the microscopy.The immunohistochemiscal staining method was used to detect aquaporin 2 (AQP2) expression intensity. Results The body weight，kidney weight and kidney index of mice in the heroin group were decreased，compared with the mice in control group.And the morphological observation showed that glomerulus pykinosis，glomerulus solid，renal tubule epithelial cells swelling necrosis and fall up.The contents for Cr and UA in plasma were higher than those in control group（P<0.01）.The activity of CAT，Na^+-K^+-ATP decreased（P<0.01），the activity of LDH，the content of MDA were higher（P<0.05 or P<0.01）and the expression of AQP2 decreased（P<0.01）. Conclusion Heroin destroys the antioxidant capacity of the kidney tissue and reduces the expression of AQP2 in the kidney and the effect of heroin on serious damage of both renal tissues structure and functions.

Key words: Heroin, Kidney tissue, Aquaporin 2, Colorimetry, Immunohistochemistry, Mouse

HE Ling-hui CHEN Cheng LI Chong-yang YU Shi-yuan. Effects of heroin on the renal tissue structures and functions of mice[J]. Acta Anatomica Sinica, 2018, 49(6): 759-764.

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