Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L4-5 spinal cord

doi:10.16098/j.issn.0529-1356.2019.03.003

Abstract

Abstract:

Objective To investigate the effect of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4-5 spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OECs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method . The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situhybridization were observed in L4-5spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L_4-5spinal cord significantly increased（P<0.05）compared with the CCI group, the reflex threshold of mechanical withdrawl of rats in the OECs group was significantly increased（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L_4-5spinal cord significantly decreased compared with the OECs group(P<0.05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher（P<0.05）. The percentage and average absorbance of P2X7 receptor positive cells in L_4-5spinal cord were lower(P<0.05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain better and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.

Key words: Olfactory ensheathing cell, Microencapsulation, Transplantation, L4-5spinal cord, Purine 2X7 receptor, In situ hybridization, Rat

ZHANG Wen-jun XIONG Mei-juan ZHOU Qi-bao SUN Yi LIU Zeng-xu. Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L4-5 spinal cord[J]. Acta Anatomica Sinica, 2019, 50(3): 280-284.

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