Effects of resveratrol on microglia polarization after oxygen glucose deprivation/reoxygenation injury in vitro

doi:10.16098/j.issn.0529-1356.2020.03.002

Abstract

Abstract:

Objective To investigate the effect of resveratrol on microglia polarization after oxygen glucose deprivation/reoxygenation (OGD/R) in vitro. Methods N9 microglias were cultured under oxygen-glucose deprivation for 150 minutes and reoxygenation for 24 hours.The experiment was divided into normal, control and resveratrol pretreatment groups. Cell viability was measured by cell counting kit-8(CCK-8) assay. Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity in the supernatant were detected by thiobarbituric acid(TBA) and water soluble terazolium salt(WST-1) respectively. Nuclear factor E2-related factor 2 (Nrf2) nuclear translocation was detected by immunofluorescence. The levels of CD206, inducible nitric oxide synthase (iNOS), Nrf2, heme oxygenase-1(HO-1), NAD(P)H: quinone oxidoreductase 1(NQO1) protein were measured by Western blotting. The mRNA levels of CD206, iNOS were detected by Real-time PCR. Results After OGD/R injury, cell viability, SOD activity, CD206, Nrf2, HO-1, NQO1 protein or mRNA expression in resveratrol groups were significantly higher than those in the control group (P<0.05, n=3), while MDA content and iNOS protein and mRNA expression were significantly lower than those in the control group (P<0.05, n=3). Nrf2 protein in resveratrol group was significantly transferred to the nucleus compared with the control group. Conclusion Resveratrol pretreatment may regulate M1/M2 polarization of microglia and reduce oxidative stress after OGD/R via strengthening activation of Nrf2.

Key words: Resveratrol, Microglia, Polarization, Nuclear factor E2-related factor 2, Oxidative stress, Western blotting, Real-time PCR

CLC Number:

LIU Jie REN Yu CHEN Yue LIU Jing XIANG Qin YANG Qin. Effects of resveratrol on microglia polarization after oxygen glucose deprivation/reoxygenation injury in vitro[J]. Acta Anatomica Sinica, 2020, 51(3): 320-325.

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