Effects of ginsenoside Re on intestinal mucosa of mice with severe acute pancreatitis and its regulation mechanism

doi:10.16098/j.issn.0529-1356.2021.04.019

Abstract

Abstract:

Objective To explore the effect and mechanism of ginsenoside Re on the intestinal mucosa of severe acute pancreatitis (SAP) mice by regulating the Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3) pathway. Methods Totally 48 mice were divided into: control group, SAP group, SAP + ginsenoside Re group and SAP+ginsenoside Re+LY2784544 group (n=12). The mice were intraperitoneally injected with caerulein solution (after fasting for 12 hours, 100 μg/kg, 6 times, injection interval 60 minutes) to establish SAP models. Mice in the SAP+ginsenoside Re group were intraperitoneally injected with ginsenoside Re (4 mg/kg, 1 time a day for 7 consecutive days). Intraperitoneal injection of 12.7 mg/kg LY2784544 was used to inhibit the JAK2/STAT3 pathway. Pancreatic and intestinal mucosal injury were detected in each group. The wet to dry weight ratio of pancreas, serum amylase and inflammatory factor levels were detected in each group. The intestinal mucosal barrier function was analyzed by detecting the levels of D-lactic acid and fluorescein isothiocyanate dextran (FITC-D). The damage of pancreatic tissue and intestinal mucosa tissue was observed by HE staining. Western blotting was used to detect the levels of apoptotic proteins Bax and Bcl-2 in the intestinal mucosa. The JAK2/STAT3 pathway expression levels in pancreatic tissue and intestinal mucosa tissue were detected by Real-time PCR and Western blotting. Results The pancreatic index, serum amylase, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α), FITC-D, D-lactic acid, Bax protein levels, JAK2 and STAT3 mRNA and protein levels in the SAP group were significantly higher than those in the control group, while Bcl-2 protein was significantly lower than that in the control group (P<0.05). The Bcl-2 protein of SAP+ginsenoside Re group was significantly higher than that of SAP group, and other indexes were significantly lower than those in SAP group (P<0.05). The Bcl-2 protein of SAP+ginsenoside Re+LY2784544 group was significantly higher than that of SAP+ginsenoside Re group, and other indexes were significantly lower than those in the SAP+ginsenoside Re group (P<0.05). Conclusion Ginsenoside Re may reduce the pancreatic injury in SAP model mice by inhibiting the JAK/STAT pathway to alleviate the inflammatory response, and may protect the small intestinal mucosal barrier by alleviating pancreatitis and inhibiting the intestinal mucosal JAK/STAT pathway to inhibit cell apoptosis.

Key words: Severe acute pancreatitis, Ginseng, Ginsenoside Re, JAK2/STAT3 pathway, Real-time PCR, Western blotting, Mouse

CLC Number:

R576

LONG Run XU Ya-pei YANG Zhu-feng YANG Jing LI Yan-hong. Effects of ginsenoside Re on intestinal mucosa of mice with severe acute pancreatitis and its regulation mechanism[J]. Acta Anatomica Sinica, 2021, 52(4): 628-634.

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