Expression of GRIM-19 and its effects in preimplantation embryo of mice

doi:10.3969/j.issn.0529-1356.2013.01.019

Abstract

Abstract:

Objective To study the expression of gene associated with retinoid-interferon-induced mortality-19(GRIM-19) and its correlation with the embryo quality, and to explore the effect of GRIM-19 in the development of preimplantation embryo of mice. Methods The expression of GRIM-19 in 2-cell, 4-cell, 8-cell, morula and blastocyst of mice preimplantation embryo was detected by using real-time PCR (n =16). Eight-cell embryos were divided into two groups: good quality embryos and bad quality embryos, according to their size, the pellucid zone and the debris of neoplasm. The different levels of embryos were detected (n=13). The expression of GRIM-19 in different groups were analyzed statistically. Results GRIM-19 expressed in every stage of preimplantation embryo of mice. The results of real-time PCR showed a gradually increase of GRIM-19 expression from 2-cell to 8-cell, and the expression then decreased progressively. In 8-cell embryo, mRNA expression of GRIM-19 in good quality embryos was significantly higher than that in bad quality embryos (P <0.05).Conclusion The expression of GRIM-19 in mouse preimplantation embryos is changed with the progress in development. GRIM-19 may reflect the potentiality of embryo development.

Key words: Gene associated with retinoid-interferon-induced mortality-19, Preimplantation embryo, Mitochondria, Real-time PCR, Mouse

CUI Wen-juan,DENG Xiao-hui,CHAO Lan*,SHEN Yan-jun,YANG Fang,FENG Wen-juan,XU Jing, CHEN Hong-lei . Expression of GRIM-19 and its effects in preimplantation embryo of mice[J]. AAS, 2013, 44(1 ): 97-101.

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