β-amyloid protein induces the changes of astrocytic N-methyl-D-aspartate receptor subunits expression in rat hippocampus

doi:10.3969/j.issn.0529-1356.2013.02.001

Abstract

Abstract:

Objective To investigate the effects of β-amyloid protein (Aβ25-35) on astrocytic N-methyl-D-aspartate receptor (NMDAR) subunits in the rat hippocampus. Methods Aβ25-35 (10μmol/L) was added into the primary cultured hippocampal cells for 1 hour and 24 hours. Immunofluorescence technique was applied to investigate the changes of the expression of NR1, NR2A and NR2B between the control group and Aβ groups ( n =10). Results NR1, NR2A and NR2B were expressed in the hippocampal astrocytes.The positive puncta were mainly distributed in the soma and process. The expression was intensive in the soma and scattered in the process. Their expression was increased following Aβ25-35treatment ( P <0.05 vs control group). The expression of NR2A and NR2B were significantly increased ( P <0.05) between Aβ 1hour group and Aβ 24hours group, while there was no significant changes in NR1 group. Conclusion The rat hippocampal astrocytes express NMDAR subunits (NR1, NR2A and NR2B), and their expressions are significantly increased after Aβ25-35treatment, while the variation tendency of NR1 is different from the changes of NR2A and NR2B.

Key words: N-methyl-D-aspartate receptor , Astrocyte , β-Amyloid protein, Hippocampus , Immunofluorescence , Rat

GAO Xiang-hong SONG Yi-zhi CHANG Li-rong ZHANG Ya-li WU Yan* . β-amyloid protein induces the changes of astrocytic N-methyl-D-aspartate receptor subunits expression in rat hippocampus[J]. AAS, 2013, 44(2 ): 146-151.

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