Abstract:

Objective To investigate the high expressed Survivin and its relationship with the apoptosis proteins in esophageal cancer of Kazakh in Xinjiang. Methods Thirty-four Survivin mRNA differential expression specimens from 52 cases of Kazakh’s esophageal cancer specimen (cancerous tissue expression in quantity than distal normal tissue) were screened. Caspase-9 and Caspase-3 gene and protein expression were detected by RT-PCR and Westernblotting respectively. Results 1. Survivin overexpressed in Kazakh’s esophageal cancer tissue, compared with distal normal tissue, differences were statistically significant (P <0.05); 2. 61.8% of the specimens had Caspase-9 low expression in 34 case specimens with Survivin mRNA differential expression, and the expression of Survivin was negatively correlated to the Caspase-9 expression（r = -0.244，P <0.05）; 3. Caspase-9 protein activation significantly decreased（P <0.05）. Conclusion The overexpression of Survivin may play an important role in the origin and development of Xinjiang Kazakh’sesophageal cancer. The function of Survivin in regulation of apoptosis may be related to direct inhibition of Caspase-9 and activation of transcription.

Key words: Esophageal cancer , Survivin , Caspase-3 , Caspase-9 , RT-PCR , Western blotting , Kazakin nationality