Expression and function of tyrosine kinase receptor KIT in mouse colonic mucosa epithelium

doi:10.3969/j.issn.0529-1356.2013.03.020

Abstract

Abstract:

Objective To investigate the expression and function of KIT in mouse colonic mucosa.Methods Western blotting and
RT-PCR techniques were used to detect the expression of c-kit gene and KIT in colonic mucosa in C57BL/6 mice (wild-type).
Immunofluorescence staining was used to examine the distribution of KIT-positive cells in colonic mucosa epithelium of C57BL/6
mice (wild-type) and N-ethyl-N-nitrosourea (ENU)-induced c-kit point mutant homozygous mice (Wads m/m).Intraperitoneal injection
of 5-bromo-2-deoxyuridin (BrdU, 30 mg/kg) was used to observe and compare the BrdU-positive cells in distal colonic mucosa epithelium
in wild-type and Wadsm/m mice, and to evaluate the effect of KIT on the renewal of colonic mucosa epithelium. Results The result of
RT-PCR indicated that c-kit gene was expressed in colonic mucosa.Western blotting showed that KIT was expressed in colonic mucosa.
Immunofluorescence staining showed that KIT-positive cells existed in the epithelium of colonic mucosa and were mainly located in
the lower part of crypts. BrdU incorporation indicated that the renewal of the epithelium of colonic mucosa in homozygous mice was
significantly slower than that in wild-type mice. Conclusion C-kit gene and KIT protein are wildely expressed in the colonic
mucosa epithelium and might be involved in the renewal of the colonic epithelium.

Key words: KIT, Colonic epithelial renewal, Colonic mucosa, Western blotting, Mouse

CLC Number:

R329.1

KANG Qian XUE Hong SUN Hai-mei LI Xiao-shuang ZHANG Guo-quan LIANG Yuan-jing* ZHOU De-shan*. Expression and function of tyrosine kinase receptor KIT in mouse colonic mucosa epithelium[J]. AAS, 2013, 44(3 ): 393-396.

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