Expression of acid-sensing ion channel 1a in endplate chondrocytes of the vertebral body in rats

doi:10.3969/j.issn.0529-1356.2013.04.006

Abstract

Abstract:

Objective To explore the expression of acid-sensing ion channels 1a(ASIC1a) in endplate chondrocytes of the vertebral body in rat. Methods Endplate chondrocytes were isolated from the 10 adult SD rats, and their phenotypes were determined by an inverted microscope and toluidine blue staining. RT-PCR and Western blotting were used to detect ASIC1a in endplate chondrocytes of the rat vertebral body. Fluorescence immunocytochemistry double marker was used to the location of ASIC1a in the cells. Results Positive staining for glycosaminoglycan was seen in endplate chondrocytes. RT-PCR analysis showed that ASIC1a mRNA were presentin endplate chondrocytes of the vertebral body. The total RNA, and the corresponding protein were detected. Conclusion The ASIC1a expresses in endplate chondrocytes of the vertebral body, which may be regarded as a new target of intervertebral disc degeneration (IVDD).

Key words: Acid-sensing ion channel, Chondrocyte, Terminal plate of vertebral body, Intervertebral disc degeneration, Rat

LI Xia ZHAO Yi-qing ZHOU Lu-yao HE Chun-hui Lü Wei-qun YUAN Feng-lai* . Expression of acid-sensing ion channel 1a in endplate chondrocytes of the vertebral body in rats[J]. AAS, 2013, 44(4 ): 475-478.

References

［1］Yuan FL, Chen FH, Lu WG, et al.Function study of acid-sensing ion channels in non-neuronal tissues［J］. Chinese Pharmacological Bulletin, 2010, 26(2): 147-150.(in Chinese)
     袁凤来, 陈飞虎, 陆伟国,等. 酸敏感离子通道在非神经组织中的功能研究［J］. 中国药理学通报, 2010, 26(2): 147-150.
［2］Yuan FL, Chen FH, Lu WG, et al. Acid-sensing ion channel 1a mediates acid-induced increases in intracellular calcium in rat articular chondrocytes［J］. Mol Cell Biochem, 2010, 340(1-2): 153-159.
［3］Lin YC, Liu YC, Huang YY, et al. High-density expression of Ca ²⁺-permeable ASIC1a channels in NG2 glia of rat hippocampus.LID-10.1371/journal.pone.0012665［doi］LID-e12665［pii］. PLoS One, 2010, 5(9). pii:e12665.
［4］Soukane DM, Shirazi-Adl A, Urban JP. Computation of coupled diffusion of oxygen, glucose and lactic acid in an intervertebral disc［J］. J Biomech, 2007, 40(12): 2645-2654.
［5］Holm S, Maroudas A, Urban JP, et al. Nutrition of the intervertebral disc: solute transport and metabolism［J］. ConnectTissue Res, 1981, 8(2): 101-119.
［6］Liu XY, Yang ShH, Xiong T, et al. Morphologic characteristics of processes of nucleus pulposus cells in adult human intervertebral disc［J］.Acta Anatomica Sinica,2007,38(4):466-469.(in Chinese)
     刘小云, 杨述华, 熊涛,等. 人椎间盘髓核细胞突起的形态学特征［J］. 解剖学报, 2007, 38(4): 466-469. 
［7］Li XY, Li KH, Song JH, et al. Comparison of morphologic characteristics of the nucleus pulposus cells from young and adult rabbit intervertebral disc［J］. Acta Anatomica Sinica, 2005, 36(6): 679-681.
     刘小云, 李康华, 宋建华,等. 幼年与成年兔椎间盘髓核细胞的形态学差异及其意义［J］.解剖学报,2005,36(6):679-681. 
［8］Walker MH, Anderson DG. Molecular basis of intervertebral disc degeneration［J］. Spine J, 2004, 4(6 Suppl): 158S-166S.
［9］Bibby SR, Jones DA, Ripley RM, et al. Metabolism of the intervertebral disc: effects of low levels of oxygen, glucose, and pH on rates of energy metabolism of bovine nucleus pulposus cells［J］. Spine (Phila Pa 1976), 2005, 30(5): 487-496.
［10］Yermolaieva O, Leonard AS, Schnizler MK, et al. Extracellular acidosis increases neuronal cell calcium by activating acidsensing ion channel 1a［J］. Proc Natl Acad Sci USA, 2004, 101(17): 6752-6757.
［11］Kusama N, Gautam M, Harding AM, et al. Acid-sensing ion channels (ASICs) are differentially modulated by anions dependenton their subunit composition［J］. Am J Physiol Cell Physiol, 2013, 304(1): C89-101.
［12］Yuan FL,Chen FH,Huang XY,et al. Expression of acid-sensing ion channels in rat articular cartilage with adjuvant arthritis［J］. Chinese Journal of Rheumatology,2008,12(5):321-324.(in Chinese)
      袁凤来, 陈飞虎, 黄学应,等. 酸敏感离子通道在大鼠佐剂性关节炎关节软骨中的表达［J］. 中华风湿病学杂志, 2008, 12(5): 321-324.

[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[3]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[4]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[5]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[6]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[7]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[8]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[9]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[10]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.
[11]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[12]	YAO Su-hua YAN Jun XIE Fang LIN Chun-hua HUANG Zheng-chun. Nerve repair effect of olanzapine-mediated cyclic AMP response element binding protein/brain-derived neurotrophic factor/receptor tyrosine kinase receptors B pathway on schizophrenic rats [J]. Acta Anatomica Sinica, 2023, 54(6): 660-667.
[13]	XU Ying LIU Bin ZHENG Xing HE Zong-zhao. Role and mechanism of complement C3a receptor in the cognitive impairment of septic rats induced by cecal ligation and perforation [J]. Acta Anatomica Sinica, 2023, 54(6): 668-675.
[14]	LI Xia ZHU Shi-jie TANG Zhong-sheng LUO Ya-fei FAN Rui-juan XIE Gao-yu KOU Yun-fang LU Ying. Mechanism of electroacupuncture regulating the ligand pathway of tyrosine kinase receptor in improving vascular dementia [J]. Acta Anatomica Sinica, 2023, 54(6): 689-694.
[15]	SUN Juan CHEN Jing-wei YANG Yi LIN Tao MOU Ya-lin ZHAO Xiu-li ZENG Guo-xi ZHANG Yan . Effects of hypoxia preconditioning on hematology-related indexes through hypoxia inducible factor-1α/stromal cell-derived factor-1 pathway in rats [J]. Acta Anatomica Sinica, 2023, 54(5): 505-511.