AAS ›› 2014, Vol. 45 ›› Issue (1): 26-30.doi: 10.3969/j.issn.0529-1356.2014.01.005

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A semi-quantitative RT-PCR analysis for NeuroD mRNA expression in brain during rat development

GUO Wei  LIU Xiao-yan  KE Li-ning  LI Yu-yu  LIN Ling  XU Jian-wen*   

  1. Department of Anatomy, Histology & Embrgology, Fujian Medical University, Center for Neuroscience Research, Fuzhou 350108, China
  • Received:2013-01-08 Revised:2013-06-05 Online:2014-02-06 Published:2014-02-06
  • Contact: XU Jian-wen E-mail:fjxjw@126.com

Abstract:

Objective To observe the NeuroD mRNA expression during rat neural system development and to investigate its possible roles in neural development and differentiation. Methods Total mRNA was abstracted from different parts of brains in different development stages with Trizol kit in rats. The mRNA of NeuroD and β-actin was reversely transcripted and amplificated by semi-quantitative reverse transcription-polymerase chain reaction method (RT-PCR). The quantity of NeuroD and β-actin mRNA expression was detected by gel imaging system. Results NeuroD mRNA existed in E7.5. The mRNA expression of NeuroD and β-actin peaked in E10.5 and E21.5, which average gray values were 20437.88±598.28 and 14482.23±1134.49, respectively. The mRNA expression of NeuroD and β-actin in E10.5 and E21.5 was significantly higher than other time points (P<0.01). The ratios of NeuroD to β-actin in E12.5,E18.5 and E21.5 were 1.59±0.09、1.61±0.07 and 1.70±0.11 respectively, which were higher than other time points(P<0.01). Conclusion The expression of NeuroD mRNA showed an apparent time-specific pattern during the development of the rat brain. The expression level of neuroD increased obviously in two phases. One was in E10.5, when the original rat brain came into being; the other was the anaphase in neural development, when brain form made the perfection, brain tissue stepped into its maturation. Thus, our results suggested the functions of NeuroD: 1. NeuroD influenced the generation and differentiation of nerve cells; 2. NeuroD played its role in the maturation of nervous system.

Key words: Neurogenic differentiation, Basic helix-loop-helix, Neuronal development, Neuronal differentiation, RT-PCR, Rat

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