Acta Anatomica Sinica

AAS ›› 2014, Vol. ›› Issue (2): 181-184.doi: 10.3969/j.issn.0529-1356.2014.02.007

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Morphological changes and NOS expression of neurons in CA3 hippocampus of rats

LIU Ji 1*WANG Xiao-yu1 SUN Yang1 GUO Xiu-ying2 PANG Yin1   

  1. 1. Hebei Cangzhou Medical College, Hebei Cangzhou 061000, China; 2. Xinhua District Institute of Health Inspection, Hebei Cangzhou 061001, China
  • Received:2013-03-07 Revised:2013-06-08 Online:2014-04-06 Published:2014-04-06

Abstract:

Objective To observe morphological changes and the nitric oxide synthase (NOS) expression of neurons in CA3 hippocampus of rats and investigate morphological changes of hippocampal neurons and the role of nitric oxide (NO) in the pathogenesis of hepatic encephalopathy. Methods Fifty male Wistar rats were divided into the control group and experimental group. Before the experiment all the rats were tested by Morris water maze test. After 9 weeks, a CCL4 model of hepatic encephalopathy was established. The hippocampus of each group was taken out for Nissl staining and dyeing of NADPH-d. Results 1.Nissl’s staining: In the experimental group, the number of neurons in hippocampus were less than the control group and dyed lighter, Nissl bodies were also reduced. 2. NADPH-d staining results: In the experimental group, the axon was dyed more deep，widespread dendrites connections were observed. In the control group there were a few positive axons and less connections between dendrites. The color in the experimental group was blue or deep blue and showed positive or strong positive with NADPH-d staining, while in the control group showed weak positive. Conclusion Hepatic encephalopathy can lead to hippocampus injury. NO may mediate the process and the blood ammonia level is one of the pathogenesis of HE.