Alteration of microglia and vasculature in the developing Tg2576 transgenic mouse

doi:10.3969/j.issn.0529-1356.2014.06.002

Abstract

Abstract:

Objective Our aim is to study the alteration of microglia and vasculature in the developing hippocampus of Tg2576 transgenic mice. Methods Tg2576 transgenic mice from postnatal day 0 (P0) to P360 were used for Iba1 and NeuN immunohistochemistry, TdT-mediated dUTP nick-end labeling (TUNEL), ink perfusion and RT-PCR analysis. Results From P180, the density of Iba1-positive cells in CA1 area of the Tg2576 transgenic mouse was significantly higher than that of the wild type mouse. The vasculature (volumetric density) of the transgenic mouse at P360 was significantly less than in the wild-type mouse (P<0.01). RT-PCR results also showed that the activity of microglia was enhanced in the AD model. Conclusion The onset and development of Alzheimer disease is correlated to the alteration of microglia and vasculature in hippocampus.

Key words: Microglia, Activation, Alzheimer’s disease, Amyloid precursor protein, Immunofluorescence, Mouse

LIU Kai NIU Yan-li LI Jin-ju WU Ping* DENG Jin-bo*. Alteration of microglia and vasculature in the developing Tg2576 transgenic mouse[J]. AAS, 2014, 45(6): 735-741.

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