Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells

doi:10.3969/j.issn.0529-1356.2014.06.016

Abstract

Abstract:

Objective To investigate the regulation mechanism of gastrodin (Gas) on atutophagy of human umbilical vein endothelial cells（HUVECs）stimulated with lipopolysaccharide (LPS). Methods Gas at 5, 10, 20 and 50μmol/L was added 1h before 1mg/L LPS stimulation, after 12h incubation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were detected by Western blottingting and RT-PCR respectively. The autophagosome was observed under fluorescent microscope by monodansylcadaverine (MDC) staining. Cell viability rate was measured by MTT analysis. Results Autophagy was induced in HUVECs by LPS-stimulation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were significantly increased,which was in a time-dependent manner. Compared with LPS-stimulated group, pertreatment with Gas inhibited the expression of autophagy in dose-dependent manner and the cell viability was effectively increased. Conclusion Autophagy was induced in HUVECs by LPS-stimulation. Gas may increase the viability rate of HUVECs through inhibiting the expression of autophagy.

Key words: Gastrodin, Lipopolysaccharide, Autophagy, Monodansylcadaverine, Western blotting, RT-PCR, Human umbilical vein endothelial cell

XIE Ya-nan YANG Ping WEN Fei SUN Lin CHEN Yuan-li* LU Di*. Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells[J]. AAS, 2014, 45(6): 824-829.

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