Abstract: Objective To observe the expressions of transforming growth factorβ(TGF-β)，aquaporin2(AQP-2) and Bax during development of renal tubules in Bufo raddei， and explore the role and regulating significance of them. Methods The expressions of TGF-β(β2，β3)， AQP-2 and Bax were observed by immunohistochemistry combined with stereological methods in Bufo raddei kidney at 1838th， young and adult incubation stages. There were 5 samples at each stage.The expression intensity of them was analyzed by IPP software. Results At 2632th stages the epithelial cells of proximal tubules and distal tubules of pronephron showed TGF-βSUB>2/SUB> immunoreactivity， at 34=38th stages the epithelial cells of proximal tubules of mesonephron showed TGF-βSUB>2/SUB> immunoreactivity and there was the same in a young and an adult Bufo raddei; At 26-30th stages the epithelial cells of proximal tubules and distal tubules of pronephron showed TGF-βSUB>3/SUB> immunoreactivity， at 32-38th stages the epithelial cells of proximal tubules of mesonephron showed TGF-βSUB>3/SUB> immunoreactivity and there was the same in a young and an adult EM>Bufo raddei/EM>; The epithelial cells of collecting tubules always appeared AQP-2 immunoreactivity at 26-38th stages and there was the same in a young and an adult EM>Bufo raddei/EM>; at 2634th stages the epithelial cells of proximal tubules and distal tubules appeared Bax immunoreac

Key words: Transforming growth factorβ, Aquaporin-2, Bax, Immunohistochemistry, Bufo raddei