Effect of epigallocatechin gallate on temporal and spatial profiles of neurons apoptosis and Caspase-3 protein expression after spinal cord injury in rats

doi:10.3969/j.issn.0529-1356.2014.01.004

Abstract

Abstract:

Objective To evaluate the effect of epigallocatechin gallate(EGCG) on temporal and spatial profiles of neuron apoptosis and its associated Caaspase-3 protein expression after spinal cord injury in rats. Methods SD rats were randomly divided into three groups (25 rats each): sham-operated group, SCI group (normal saline solution intraperitoneal injection, immediately and 1 hour after SCI), and EGCG treatment groups (50mg/kg, intraperitoneal injection, immediately and 1 hour after SCI ). SCI model was made by clamps methods. The rats were sacrificed at 6, 12, 24 hours, 3 and 7days; 5 rats at each time point after injury. Spinal cord tissue in the injured area was harvested, sectioned 0-5.00 mm rostral from the epicenter post-injury and examined by cresyl violet (CV) staining and terminal-deoxynucleoitidyl transferase (TdT)-mediated deoxy uridine triphosphate-biotin nick end labeling (TUNEL) staining method and immunohistochemical method. Results At all the time points observed, CV-staining result showed that the neurons was not found in section 0-0.50 mm for SCI and EGCG group, the neurons was increased in EGCG group than in the SCI group in the sections 1.00-4.60 mm. The number of TUNEL positive neurons and Caspase-3 neurons were decreased in EGCG group than in SCI group in the section 1.05-3.05 mm and 1.10-3.10 mm rostral from the epicenter. Conclusion EGCG can decrease the expression of Caspase-3 and inhibit neuronal cell apoptosis in the rats after spinal cord injury on temporal and spatial profiles, which may play a significant neuroprotective role in the secondary SCI.

Key words: Spinal cord injury, Epigallocatechin gallate, Apoptosis, Caspase-3, TUNEL, Rat

LIU Xin-ning DIAO Yao* LAN Zhen-he MA Jing GE Rui JIAO Ying ZHU Yue. Effect of epigallocatechin gallate on temporal and spatial profiles of neurons apoptosis and Caspase-3 protein expression after spinal cord injury in rats[J]. AAS, 2014, 45(1): 20-25.

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