Expression of tumor stem cell markers in the tumor sphere from esophageal squamous cells carcinoma Eca109

doi:10.3969/j.issn.0529-1356.2014.03.010

Abstract

Abstract:

Objectiv To observe the expression of tumor stem cell markers P75NTR,Oct-4,Sox-2,Lin28 and Nanog in the tumor sphere from esophageal squamous cells carcinoma Eca109 and identify the esophageal squamous cell cancer stem cell marker. Methods The serum-free culture method was used for generating tumor spheres: proliferation was observed in enrichment culture tumor spheres. Small tumor spheres were obtained after 5 days culture and big and round tumor spheres appeared after 14 days culture which were collected for experiments and passaged.The expression and location of P75NTR,Oct-4,Sox-2,Lin28, and Nanog were detected by immunofluorescence cytochemistry. Results The expressions of P75NTR,Oct-4 and Lin28 were positive in the center of tumor spheres and some on cytoplasm and other in nuclei of Eca109 monolayer cells. However, Oct-4 fluorescence intensity was weaker than P75NTR.The expressions of Sox-2 and Nanog were positive in cytoplasm of tumor spheres and Eca109 monolayer cells. Conclusion The cells expressing P75NTR, Oct-4, and Lin28 in the center of the tumor sphere may be esophageal cancer stem cells.

Key words: P75NTR, Oct-4, Sox-2, Lin28, Nanog, Cancer stem cell, Esophageal squamous cell carcinoma, Tumor sphere formation

HUANG Yan-yan PEI Xue-lian LIU Yang LI Qiu-xia WANG Yu-ting MU Xiao-ling*. Expression of tumor stem cell markers in the tumor sphere from esophageal squamous cells carcinoma Eca109[J]. AAS, 2014, 45(3): 350-353.

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