Effects of basic fibroblast growth factor on endothelial function and structure of the basilar artery of atherosclerotic rats

doi:10.3969/j.issn.0529-1356.2014.04.006

Abstract

Abstract:

Objective To study the protective effect of basic fibroblast growth factor (bFGF) on endothelial function and structure of the basilar artery of atherosclerotic rats. Methods A total of forty-eight male adult Wister rats were randomly divided into the normal control, the atherosclerosis (AS) model and the bFGF treatment groups. The AS model group and the bFGF treatment group were injected with a single dose of vitamin D3 (6×105IU/kg) and loaded with high fat diet for six consecutive weeks. The bFGF (9.5μg/kg, twice one day) was injection into the abdominal cavity after six weeks in the bFGF treatment group for two weeks, and an identical volume saline was given for the AS model group and the normal control group. After eight weeks, all the rats were sacrificed. The relaxation percentages of the isolated basilar artery in response to acetylcholine (Ach) were detected and the pathological lesions of them were observed under a light microscope. ELISA and colorimetry assayed the content of serum VEGF and basilar arterial nitric oxide (NO). The basilar artery was used for primary culture of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). The influence of bFGF on the proliferation vitality of VECs was measured in vitro with MTT assay. TRITC-phalloidin labeling the cytoskeleton microfilament of VSMCs was observed by laser confocal microscopy. Results The early AS plaques were presented after six weeks by hyper lipid foods. Compared with the AS model group, the relaxation percentage of the isolated basilar artery, the content of both serum VEGF and basilar arterial NO in the bFGF treatment group were obviously increased, but the pathologic injury of the basilar artery was significantly decreased (P<0.05). The proliferation vitality of VECs was obviously increased (P<0.05); the cytoskeleton microfilament of VSMCs was of obviously improvement. Conclusion AS may aggravate the basilar arterial injury, but bFGF may efficiently improve the arterial endothelial function and decrease the pathological lesion of the basilar artery in the AS model rats, which may promote the arterial protective effect.

Key words: Basic fibroblast growth factor, Atherosclerosis；Basilar artery, Vascular endothelial cells, Smooth muscle cells, Enzyme-linked immunosorbent assay, Rat

ZHU Jun-de* YU Yan GE Guo. Effects of basic fibroblast growth factor on endothelial function and structure of the basilar artery of atherosclerotic rats[J]. AAS, 2014, 45(4): 469-474.

References

［1］Hergenreider E, Heydt S, Tréguer K, et al. Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs［J］. Nat Cell Biol, 2012,14 (3):249-256. ［2］Kawano N, Emoto M, Mori K, et al. Association of endothelial and vascular smooth muscle dysfunction with cardiovascular risk factors, vascular complications, and subclinical carotid atherosclerosis in type 2 diabetic patients［J］. J Atheroscler Thromb, 2012,19 (3):276-284.
［3］Koon CM, Woo KS, Leung PC, et al. Salviae miltiorrhizae radix and puerariae lobatae radix herbal formula mediates anti-atherosclerosis by modulating key atherogenic events both in vascular smooth muscle cells and endothelial cells ［J］. J Ethnopharmacol, 2011, 138(1):175-183. 
［4］Matsuse D, Kitada M, Ogura F, et al. Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and basic fibroblast growth factor releasing microspheres enhances recovery after cerebral ischemia in rats［J］. Tissue Eng Part A, 2011, 17(15-16):1993-2004.
［5］Zhu JD, Wang GX, Yu Y, et al. Effect of transplantation with bone marrow-derived endothelial progenitor cells on learning and memory and neuron in the cortex of parietal lobe after cerebral ischemia ieperfusion injury of Atherosclerotic model rats ［J］. Acta Anatomica Sinica, 2012, 43 (4): 433-438. (in Chinese)
朱俊德，王贵学，余彦，等. 内皮祖细胞移植对动脉粥样硬化模型大鼠脑缺血再灌注后学习记忆能力与脑顶叶皮质的影响［J］. 解剖学报, 2012, 43(4): 433-438.
［6］Xu AD, Zhou WY, Lin XH, et al. Asymptomatic intracranial arterial stenoses in patients with hypertension［J］. Journal of Hypertension, 2004,12(2): 123-126. (in Chinese)
徐安定, 卓文燕, 林秀华, 等. 高血压病人无症状性颅内动脉狭窄的分布特征和危险因素［J］.高血压杂志，2004,12(2): 123-126.
［7］Feldmann E, Wilterdink JL, Kosinski A, et al. The stroke outcomes and neuroimaging of intracranial atherosclerosis (SONIA) trial［J］. Neurology, 2007, 68(24):2099-2106.
［8］Bae HJ, Lee J, Park JM, et al. Risk factor of intracranial cerebral atherosclerosis among asymptomatics ［J］. Cerebrovasc Dis, 2007, 24 (4): 355-360.
［9］Cruz-Flores S, Rabinstein A, Biller J, et al. Racial-ethnic disparities in stroke care: the American experience: a statement for healthcare professionals from the American Heart Association/American Stroke Association ［J］. Stroke,2011,42(7):2091-2116.
［10］Liu Y, Shuai J, Zhen J, et al. Clinical diagnosis and endovascular stenting of symptomatic carotid and vertebral artery stenosis ［J］. Journal of Apoplexy and Nervous Diseases, 2006, 23(6): 696-698. (in Chinese)
刘勇, 帅杰, 郑健, 等. 症状性颈、椎动脉狭窄的临床诊断与血管内介入治疗［J］. 中风与神经疾病杂志,2006, 23(6): 696-698.
［11］Barnet HJ. Carotid disease and cognitive dysfunction ［J］. Ann Intern Med, 2004, 140(4):303-304.
［12］Aktas RG, Kayton RJ. Ultrastructural immunolocalization of basic fibroblast growth factor in endothelial cells: morphologic evidence for unconventional secretion of a novel protein ［J］. J Mol Histol, 2011, 42(5):417-425.
［13］Mosmann T. Rapid colorimetric assay for cell growth and survival: application to proliferation and cytotoxicity assay［J］. Immunol Methods, 1983, 65(1-2): 55-63.
［14］Ryoo S, Berkowitz DE, Lim HK. Endothelial arginase Ⅱ and atherosclerosis［J］. Korean J Anesthesiol, 2011,61(1):3-11.
［15］Lange K, Gartzke J. F-actin-based Ca signaling-a critical comparison with the current concept of Ca signaling ［J］. J Cell Physiol, 2006,209(2): 270-287.



[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[3]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[4]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[5]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[6]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[7]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[8]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[9]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[10]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.
[11]	CHEN Zi-xuan SI Li-na SHU Wei-han ZHANG Xin WEI Chen-yang CHENG Lu-yang YANG Song-he QIAO Yue-bing. Mechanism of melatonin regulation of PI3K/Akt/mTOR signaling in hypothalamus delaying the initiation of puberty in female mice mice [J]. Acta Anatomica Sinica, 2023, 54(6): 644-651.
[12]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[13]	YAO Su-hua YAN Jun XIE Fang LIN Chun-hua HUANG Zheng-chun. Nerve repair effect of olanzapine-mediated cyclic AMP response element binding protein/brain-derived neurotrophic factor/receptor tyrosine kinase receptors B pathway on schizophrenic rats [J]. Acta Anatomica Sinica, 2023, 54(6): 660-667.
[14]	XU Ying LIU Bin ZHENG Xing HE Zong-zhao. Role and mechanism of complement C3a receptor in the cognitive impairment of septic rats induced by cecal ligation and perforation [J]. Acta Anatomica Sinica, 2023, 54(6): 668-675.
[15]	LI Xia ZHU Shi-jie TANG Zhong-sheng LUO Ya-fei FAN Rui-juan XIE Gao-yu KOU Yun-fang LU Ying. Mechanism of electroacupuncture regulating the ligand pathway of tyrosine kinase receptor in improving vascular dementia [J]. Acta Anatomica Sinica, 2023, 54(6): 689-694.