Comparison of glucose and lipid metabolism in two types of Parkinson’s disease rat models

doi:10.3969/j.issn.0529-1356.2014.05.004

Abstract

Abstract:

Objective To select an ideal Parkinson’s disease (PD) animal model with metabolic abnormalities for subsequent experimental studies. Methods A total of 62 Sprague-Dawley male rats were randomly divided into four groups: damaged medial forebrain bundle (MFB) model group, damaged medial forebrain bundle (MFB) sham group, damaged Striatum model group and damaged Striatum sham group. After detecting the rotation experiment, successful model rats of two groups were selected to detect the changes of food intake, body weight, blood glucose and intra-abdominal adipose tissue. Results It was easier to produce a PD model by destroying MFB than striatum. Compared with sham-operated rats, MFB model rats showed significant abnormality both in reduction of body weight ［(218.1±13.99)g vs (252.7±10.1)g, P<0.05］ and high blood glucose appeared at 15min and 30min after introperitoneal glucose tolerance test (IPGTT). Their perirenal white adipose tissue was significantly reduced (both left and right side). Striatum model rats only appeared decreased food intake ［(13.95±0.25)g vs (20.23±0.86)g, P<0.001］ and impaired glucose regulation at 15min, 30min and 60min after IPGTT. Their body weight and adipose tissue did not change significantly. Conclusion No matter in the success rate or metabolismrelated indicators, MFB damaged rat model of PD is more suitable to study PD patients with abnormal lipid metabolism compared with Striatum rat model.

Key words: Parkinson disease, Glucose and lipid metabolism, 6-hydroxydopamine, Immunohistochemistry, Rat

MENG Xiang-zhi ZHENG Rui-pan ZHANG Ying QIAO Ming-liang JING Peng GAO Yan*. Comparison of glucose and lipid metabolism in two types of Parkinson’s disease rat models[J]. AAS, 2014, 45(5): 605-609.

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