Neuroprotective effect of grafting thyroid hormone-induced neural stem cells on chronic experimental allergic encephalomyelitis

doi:10.16098/j.issn.0529-1356.2015.01.003

Abstract

Abstract:

Objective To explore whether transplantation of neural stem cells induced by thyroid hormone (T3) provides a better neuroprotective effect than native NSCs after chronic experimental allergic encephalomyelitis. Methods Neural stem cells (NSCs) and T3-induced NSCs of neonatal rat were cultured in vitro. After 7 days they were induced to differentiate, immunohistochemistry or immunofluorescence staining were used to detect GalC+ and GFAP+ cells respectively. Chronic EAE rat models were induced by Guinea pig spinal cord homogenate, fo11owed by infusion of T3/NSCs, NSCs (T3/NSCs and NSCs were labeled with 5-bromo-2’-deoxyuridine (BrdU) and saline (T3/NSCs, NSCs and control groups，respectively) at 10 days after EAE immunization, and each group had 10 rats. Clinical scores for every day were performed to evaluate neuro1ogica1 function. All rats were sacrificed at 60d after EAE immunization. HE and LFB staining was used to observe inflammatory infiltrates and demyelination in brain respectively. Immunofluorescent double staining was used to test the ratio of GalC⁺/BrdU⁺ and GFAP⁺/BrdU⁺in brain. RT-PCR assay was used to identify the expression of mRNA for PDGFαR, GalC and MBP of brain tissue. Results The ratio of GalC⁺ or GFAP⁺ cells of T3/NSCs was respectively higher or lower than that of NSCs in vitro. Significant recovery of neuro1ogica1 function was found in T3/NSCs and NSCs groups compared with control group. Improvement of inflammatory infiltrates and demyelination in T3/NSCs groups was stronger than that in NSCs groups. In brain, the ratio of GalC+/BrdU⁺ or GFAP⁺/BrdU⁺ in T3/NSCs group was respectively higher or lower than that in NSCs group. And the expression of mRNA for PDGFαR, GalC and MBP of brain tissue in T3/NSCs groups was stronger than in NSCs groups. Conclusion The results demonstrate that grafting T3-induced NSCs provides better neuroprotection for EAE than grafting NSCs alone.

Key words: Thyroid hormone, Neural stem cell, Cell transplantation, Allergy, Encephalomyelitis, Immunofluorescence, Rat

DU Jie HU Guang-qiang DENG Li YANG Chao-xian GUO Kan GAO Xiao-qing*. Neuroprotective effect of grafting thyroid hormone-induced neural stem cells on chronic experimental allergic encephalomyelitis[J]. AAS, 2015, 46(1): 13-19.

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